PE Rabbit anti-Human CD156c/ADAM10 Monoclonal Antibody for Flow Cytometry - O14672

Core Product Specifications and Parameters

Biological Background: ADAM10 Function and Localization

• ADAM10 (Disintegrin and metalloproteinase domain-containing protein 10), also known as CD156c and Kuzbanian, is a transmembrane metalloprotease belonging to the ADAM family.
• It functions as a major ectodomain sheddase for a wide range of adhesion proteins, growth factor precursors, and cytokines, playing essential roles in development and tissue homeostasis. Related references: PMID:11786905, PMID:12475894, PMID:20592283
• ADAM10 cleaves the membrane-bound precursor of tumor necrosis factor (TNF) and is responsible for the constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP), linking it to inflammation and Alzheimer's disease pathology. Related references: PMID:11786905, PMID:26686862, PMID:29224781
• It mediates the proteolytic release of cellular prion protein and adhesion molecule L1, and controls Notch receptor processing, which is critical for neurogenesis and immune cell development. Related references: PMID:11477090, PMID:12475894
• ADAM10 sheds FasL, generating a transmembrane signaling remnant, and cleaves the immune checkpoint receptor LAG3, influencing immune regulation. Related references: PMID:17557115
• The protein localizes to the cell membrane, Golgi apparatus, cytoplasmic vesicles, and adherens junctions, with expression detected in the brain, spleen, lymph nodes, and peripheral blood leukocytes.
• As a zinc-dependent metalloprotease, ADAM10 is characterized by disulfide bonds, glycosylation, and phosphorylation, and its dysregulation is associated with Alzheimer disease and amyloidosis.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is suitable for direct flow cytometry to detect surface ADAM10 on live human cells.
• We recommend titrating the antibody to determine the optimal concentration for your specific cell type and assay conditions.
• Include appropriate isotype control and Fc receptor blocking to minimize non-specific binding.
• If intracellular staining is required, validate fixation and permeabilization protocols for compatibility with PE fluorescence.
• Confirm ADAM10 expression in your target cell population using known positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with a comprehensive one-stop channel for IVD raw materials, technical services, and consulting. For research involving ADAM10 in neuroinflammation, oncology, or immunology, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, recombinant proteins, and bulk ancillary reagents. Our global network enables efficient sourcing of rare and specialized raw materials to accelerate your project from concept to clinic.