PE Rabbit anti-Human CD153 mAb - P32971

Core Product Specifications and Parameters

Biological Background: CD153 Function and Localization

• CD153, also known as tumor necrosis factor ligand superfamily member 8 (TNFSF8) or CD30 ligand (CD30-L), is a type II transmembrane protein belonging to the TNF superfamily.
• It binds specifically to the receptor TNFRSF8/CD30, triggering downstream signaling pathways that induce proliferation of T-cells.
• As a member of the TNF ligand family, CD153 shares structural features such as a TNF homology domain that mediates receptor binding and trimerization.
• CD153 is predominantly localized on the cell membrane as a single-pass transmembrane protein, with its C-terminal TNF homology domain exposed extracellularly.
• The protein contains a signal-anchor transmembrane helix and undergoes post-translational modifications including glycosylation and disulfide bond formation, which are critical for its stability and function.
• CD153 is part of the reference proteome and has been identified in proteomics studies, reflecting its relevance in immune cell communication.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 63-234), making this antibody suitable for detecting native surface CD153 by flow cytometry.
• As a PE-conjugated primary antibody, it enables direct single-step staining; ensure proper titration and use of isotype controls to minimize background.
• For cell surface staining, prepare single-cell suspensions and follow standard protocols for live-cell flow cytometry, avoiding fixation that may alter epitope recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like this PE-conjugated anti-CD153 clone, supporting T-cell biology and lymphoma flow cytometry applications. Our supply chain covers bulk ancillary reagents and rare target sourcing, enabling diagnostic manufacturers and research labs to accelerate development from concept to clinic. Count on CamelBio for reliable procurement and technical guidance across your entire project lifecycle.