PE Rabbit anti-Human CD146 Monoclonal Antibody for FC - P43121

Core Product Specifications and Parameters

Biological Background: Cell surface glycoprotein MUC18 Function and Localization

• Cell surface glycoprotein MUC18, also known as MCAM or CD146, is a membrane adhesion molecule belonging to the immunoglobulin superfamily.
• It plays a critical role in cell-cell adhesion, maintaining endothelial monolayer cohesion at intercellular junctions in vascular tissue.
• Its expression on melanoma cells facilitates interactions with the vascular system, promoting hematogenous tumor spread and metastasis.
• Functions as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, along with a transient increase in intracellular calcium concentration.
• Predominantly localized to the membrane; detected in endothelial cells throughout the body and in vascular smooth muscle of normal adult tissues.
• Associated with tumor progression: strongly expressed on metastatic lesions and advanced primary melanomas, but rarely on benign nevi or thin primary tumors.
• Features include a signal peptide, extracellular immunoglobulin-like repeats, a transmembrane helix, and is subject to glycosylation, phosphorylation, and disulfide bond formation.
• Alternative splicing produces multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 24-559 of human CD146 (extracellular region), ensuring recognition of native surface protein in flow cytometry applications.
• Consider titrating the antibody to determine the optimal staining concentration for your specific cell type, and always include an appropriate isotype control.
• CD146 expression is characteristic of endothelial cells, melanoma cells, certain lymphocytes, and mesenchymal stem cells; verify target expression in your experimental system.
• The PE conjugate provides bright fluorescence suitable for multicolor flow cytometry panels; ensure compatible instrument settings and compensation.
• Store and handle the antibody under recommended conditions (ice bag shipping) to preserve conjugate stability and binding activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, offering diagnostic manufacturers, labs, and research institutes streamlined access to a comprehensive portfolio of critical reagents. For researchers and developers focusing on endothelial biology, oncology, and cell adhesion, this PE anti-CD146 antibody exemplifies the high-quality monoclonal antibodies available for assay development. Beyond single reagents, CamelBio supplies validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing—bridging your entire workflow from concept to clinic.