PE Rabbit anti-Human CD141/Thrombomodulin mAb - P07204

Core Product Specifications and Parameters

Biological Background: Thrombomodulin (CD141) Function and Localization

• Thrombomodulin (CD141), also known as fetomodulin, is encoded by the THBD gene (alternative name THRM) and serves as an endothelial cell receptor with critical roles in hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis. Related references: PMID:10761923

• It acts as a cofactor for thrombin-mediated activation of protein C on the vascular endothelial surface, initiating the anticoagulant pathway. Related references: PMID:29323190, PMID:33836597, PMID:9395524

• Thrombomodulin accelerates the activation of plasma carboxypeptidase B2/CPB2, which removes C-terminal basic amino acids from substrates such as kinins and anaphylatoxins, thereby inhibiting fibrinolysis. Related references: PMID:26663133

• It modulates protease-activated receptor 1 (PAR1) cleavage specificity, inhibiting endothelial cell permeability and inflammation.

• The protein suppresses inflammation independently of its anticoagulant role by sequestering HMGB1, preventing its interaction with RAGE and other inflammatory receptors. Related references: PMID:15841214

• Thrombomodulin is uniquely expressed on endothelial cells and is localized to the membrane as a single-pass transmembrane glycoprotein.

• Structural features include multiple disulfide bonds, EGF-like domains, and a proteoglycan component; post-translational modifications include hydroxylation and glycosylation.

• Mutations in THBD are associated with atypical hemolytic uremic syndrome (aHUS) and thrombophilia, highlighting its critical role in vascular hemostasis.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is supplied for flow cytometry (FC) applications. For initial experiments, titrate the antibody over a recommended concentration range (e.g., 0.25–2 µg per test) to determine optimal staining conditions for your specific cell type.
• The immunogen corresponds to the extracellular domain (amino acids 19-515) of human CD141/Thrombomodulin, suggesting recognition of the soluble and membrane-bound forms. Validate reactivity in the relevant human endothelial or immune cell subsets known to express CD141 (e.g., BDCA-3+ dendritic cells).
• For multicolor flow cytometry panels, ensure proper compensation using single-stained controls, particularly when combining with other PE-labeled or tandem dyes.
• Because thrombomodulin expression may be modulated by inflammatory stimuli, consider using appropriate activation controls (e.g., TNF-alpha treatment) when assessing expression levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material and technical services, covering every stage from concept to clinic. As a sourcing partner, we provide validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom reagent development to support your coagulation, hemostasis, and immunotherapy research. This PE-labeled anti-CD141/Thrombomodulin antibody exemplifies our commitment to supplying high-quality, application-tested reagents for diagnostic and translational studies.