PE Rabbit anti-Human CD13/ANPEP Monoclonal Antibody for FC - P15144

Core Product Specifications and Parameters

Biological Background: CD13/ANPEP Function and Localization

• CD13/ANPEP (Aminopeptidase N) is a zinc-dependent metalloprotease that cleaves neutral or basic amino acids from the N-terminus of peptides, playing a key role in the final digestion of peptides in the brush border of the small intestine and kidney.
• It processes various bioactive peptides including angiotensin III and IV, neuropeptides, and chemokines, thereby regulating blood pressure, neural signaling, and immune responses.
• CD13 is implicated in angiogenesis and cholesterol crystallization, and it may promote tumor growth and metastasis by facilitating endothelial cell migration and invasion.
• The protein serves as a receptor for human coronavirus 229E (HCoV-229E) and mediates human cytomegalovirus (HCMV) infection, highlighting its role in host-pathogen interactions.
• Subcellularly, CD13 is a type II transmembrane glycoprotein located primarily on the cell membrane, with a large extracellular domain containing the catalytic site.
• Tissue expression is broad: it is found on epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, and pericytes; also in synovial fibroblasts and activated lymphocytes. A soluble form is present in plasma and is elevated in cancer patients.
• Post-translational modifications include N-glycosylation, disulfide bond formation, and sulfation; the protein is also phosphorylated, which may regulate its activity.

Experimental Guidance and Technical Tips

• This PE-conjugated monoclonal antibody is intended for flow cytometry. When setting up multicolor panels, consider titration to optimize staining and avoid spectral overlap with other PE-labeled antibodies.
• For intracellular staining, permeabilization may be required as the target is a transmembrane protein.
• Validate the antibody in the relevant sample system, such as human peripheral blood mononuclear cells or cell lines expressing CD13 (e.g., HL-60, U937).
• The antibody is reported to react with human CD13; cross-reactivity with other species has not been determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and life science laboratories with a comprehensive portfolio of high-quality antibody raw materials, including monoclonal antibodies, recombinant antigens, and ancillary reagents. This PE-conjugated anti-CD13 monoclonal antibody is part of our expanding catalog for immunology and infectious disease applications, backed by rigorous quality control. For your specific project, CamelBio can source validated antibody pairs, bulk quantities, and custom conjugations to streamline your assay development.