PE Rabbit anti-Human CD124/IL-4Rα Monoclonal Antibody for Flow Cytometry - P24394

Core Product Specifications and Parameters

Biological Background: CD124/IL-4Rα Function and Localization

• CD124 (IL-4Rα) is a receptor subunit for both interleukin 4 (IL-4) and interleukin 13 (IL-13), playing a central role in type 2 immune responses. Related references: PMID:17030238
• Upon ligand binding, it couples to the JAK1/2/3-STAT6 signaling cascade, driving transcriptional programs associated with Th2 cell differentiation.
• In B cells and other cell types, IL-4/IL-13 signaling through IL-4Rα regulates IgE class switching and production, as well as chemokine and mucus secretion at sites of allergic inflammation.
• In certain cell contexts, IL-4Rα can also signal via insulin receptor substrates IRS1 and IRS2, linking cytokine signaling to metabolic pathways.
• A soluble isoform (sIL4R) is produced by alternative splicing and acts as a decoy receptor, inhibiting IL-4-mediated proliferation and IL-5 upregulation in T cells.
• The receptor is primarily localized to the cell membrane, with the soluble form being secreted; it is highly expressed in activated T-cells (isoforms 1 and 2).
• Post-translational modifications include glycosylation and phosphorylation, and the protein contains disulfide bonds and a single-pass transmembrane domain.

Experimental Guidance and Technical Tips

• The immunogen used (recombinant protein covering amino acids 24-232) targets the extracellular domain; consider this when designing complementary detection reagents.
• For flow cytometry, titrate the antibody to determine the optimal dilution in your cell system and include appropriate isotype controls.
• Validate staining specificity using IL-4Rα-negative cells or blocking with unconjugated antibody.
• Avoid prolonged light exposure to preserve the PE fluorophore during sample preparation and acquisition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk reagents. For studies of CD124/IL-4Rα in Th2 differentiation and allergic inflammation, we offer targeted reagents and can assist in procuring rare raw materials. Our dedicated team supports you from concept to clinic.