PE Rabbit anti-Human CD123 mAb - P26951

Core Product Specifications and Parameters

Biological Background: CD123 Function and Localization

• Interleukin-3 receptor subunit alpha (IL3RA, CD123) is the ligand-specific alpha chain of the high-affinity IL-3 receptor, essential for hematopoietic cell proliferation and differentiation. Related references: PMID:10527461
• Upon IL-3 binding, CD123 heterodimerizes with the common beta subunit (IL3RB) to activate intracellular signaling cascades, including the JAK2/STAT5 pathway, which promotes cell survival and lineage commitment.
• CD123 is primarily expressed on the surface of hematopoietic progenitor cells, monocytes, and B-lymphocytes, positioning it as a key marker in hematopoiesis and myeloid malignancies.
• The protein is localized to the cell membrane, with structural features typical of a type I transmembrane receptor, including an extracellular ligand-binding domain, a single transmembrane helix, and a cytoplasmic tail.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, and ubiquitination, which may regulate receptor expression, stability, and signaling.
• The IL3RA gene undergoes alternative splicing, producing multiple isoforms that may have distinct functional roles or tissue-specific expression patterns.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is suitable for direct flow cytometric detection of CD123 on live, unfixed human hematopoietic cells. Use appropriate isotype controls to assess background staining.
• The immunogen corresponds to the extracellular domain (residues 1–378), suggesting the antibody likely recognizes an epitope within the N-terminal ligand-binding region. The antibody may compete with IL-3 binding; users should validate performance in functional assays if blocking activity is suspected.
• For optimal results, titrate the antibody concentration on the target cell population of interest, as staining intensity can vary with receptor density. Common positive controls include acute myeloid leukemia (AML) cell lines or peripheral blood monocytes.
• As with any fluorochrome-conjugated antibody, protect from light and use within the recommended storage conditions. Avoid freeze-thaw cycles to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with end-to-end access to high-quality IVD raw materials, from concept to clinic. For CD123 and hematopoietic research, we offer a portfolio that includes validated monoclonal antibodies, recombinant proteins, and ancillary reagents tailored for immunophenotyping and leukemia diagnostic development. Our sourcing expertise extends to rare targets, ensuring reliable supply for your assay building needs.