PE Rabbit anti-Human CD101/IGSF2 Monoclonal Antibody for FC - Q93033

Core Product Specifications and Parameters

| Product Name | PE Rabbit anti-Human CD101/IGSF2 mAb | | Remarks/Alias | V7; IGSF2; EWI-101 | | Species | Human | | Gene ID (Human) | 9398 | | Gene ID | 9398 | | Immunogen | Recombinant protein | Recombinant fusion protein containing a sequence corresponding to amino acids 21-410 of human CD101/IGSF2 (NP_004249.2) | | Source | Rabbit | | Category | Monoclonal Antibodies | | Application | FC | | Cross Reactivity | Human | | SWISS | Q93033 | | Protein Weight | 115kDa | | Shipping | Ice bag |

Biological Background: CD101/IGSF2 Function and Localization

• Protein identity: Immunoglobulin superfamily member 2 (CD101), also known as cell surface glycoprotein V7, Glu-Trp-Ile EWI motif-containing protein 101 (EWI-101).
• Core function: Acts as an inhibitor of CD3-induced T-cell proliferation, suppressing IL2RA expression, IL2 secretion, and tyrosine kinase activity.
• Inhibitory mechanism: Prevents PLCG1 phosphorylation, intracellular calcium flux, and nuclear translocation of NFAT.
• May contribute to T-cell inhibition via IL10 secretion by cutaneous dendritic cells, linking innate and adaptive immunity.
• Gene family and structure: Member of the immunoglobulin superfamily, containing immunoglobulin domains, a signal peptide, and a transmembrane domain. Keywords: Disulfide bond, Glycoprotein, Repeat, Signal, Transmembrane helix.
• Subcellular localization: Membrane (single-pass type I membrane protein).
• Tissue and cellular expression: Highly expressed in lung, thymus, and small intestine; detected on cutaneous dendritic cells, activated T-cells, monocytes, granulocytes, and epithelial cells with dendritic morphology.
• Clinical relevance: Expressed on CD4+ CD56+ leukemic tumor cells and Langerhans cells from Langerhans cell histiocytosis patients, suggesting a potential diagnostic marker.

Experimental Guidance and Technical Tips

• The PE conjugation allows direct flow cytometry detection without secondary antibodies, simplifying experimental workflows.
• For optimal staining, consider titrating the antibody on your cell type of interest under standard FC conditions.
• Given the target's expression on activated T-cells and dendritic cells, validate specificity using appropriate positive and negative controls (e.g., resting vs. activated T-cells).
• Ensure the cell processing protocol maintains surface protein integrity, as CD101/IGSF2 is a transmembrane protein.
• If using for intracellular detection, note that the immunogen corresponds to the extracellular domain; thus, it is expected to recognize surface CD101 primarily.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to critical IVD raw materials. As your sourcing partner, we support projects targeting T-cell regulation and cancer immunology, offering validated antibody pairs, bulk reagents, and custom development services to accelerate assay development. Our portfolio includes optimized monoclonal antibodies like this anti-CD101 clone to ensure reliable performance in flow cytometry applications.