PE Rabbit anti-Human CD100/SEMA4D Monoclonal Antibody for Flow Cytometry - Q92854

Core Product Specifications and Parameters

Biological Background: Semaphorin-4D Function and Localization

• Semaphorin-4D (CD100) is a transmembrane glycoprotein belonging to the semaphorin family; it exists also as a secreted form generated by proteolytic cleavage.
• Located on the cell membrane and secreted into the extracellular space.
• Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus; lower expression in brain, kidney, testis, and other tissues.
• In the immune system, induces B-cell aggregation and enhances their viability in vitro. Related references: PMID:8876214
• Promotes migration of cerebellar granule cells during neuronal development. Related references: PMID:16055703
• Modulates neurite arborization and complexity in hippocampal neurons via PLXNB1 and RHOA activation. Related references: PMID:19788569
• Stimulates endothelial cell migration through the PTK2B/PYK2, SRC, and phosphatidylinositol 3-kinase-AKT pathway. Related references: PMID:16055703
• Functions as a cell surface receptor for PLXNB1 and PLXNB2, mediating cell-cell signaling. Related references: PMID:20877282

Experimental Guidance and Technical Tips

• The PE conjugate allows direct detection in flow cytometry without secondary reagents; titrate the antibody to determine optimal concentration for your cell system.
• As the immunogen covers the extracellular domain (aa 22–734), the antibody is expected to recognize the native surface receptor on live cells.
• Include appropriate isotype controls and live/dead viability staining to ensure specific signal detection.
• Fixation and permeabilization may alter the epitope; validate under your specific assay conditions.
• The reported protein weight range (82–96 kDa) may reflect differential glycosylation; consider this when performing SDS-PAGE under reducing/non-reducing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials and technical services from concept to clinic. This anti-CD100/SEMA4D antibody is suitable for flow cytometry applications in immunology and neuroscience research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare-target raw materials to streamline your development pipeline.