PE Rabbit anti-Human CaSR Monoclonal Antibody for FC - P41180

Core Product Specifications and Parameters

Biological Background: CaSR Function and Localization

• CaSR (extracellular calcium-sensing receptor) is a G protein-coupled receptor that senses extracellular calcium concentration and is critical for calcium homeostasis. Related references: PMID:17555508, PMID:19789209, PMID:21566075
• It is primarily expressed in parathyroid glands and kidneys, where it regulates PTH secretion and renal calcium resorption, respectively.
• CaSR signals through different G proteins: G(q)/G(11) activates phospholipase C-beta, while G(i)/G(o) inhibits adenylate cyclase. Related references: PMID:38632411, PMID:7759551
• The receptor is activated by a co-agonist mechanism involving aromatic amino acids (Trp/Phe) and divalent cations (calcium/magnesium). Related references: PMID:27386547, PMID:27434672, PMID:32817431
• CaSR acts as an activator of the NLRP3 inflammasome via G(i)/G(o)-mediated downregulation of cAMP. Related references: PMID:32843625
• Subcellular location: Cell membrane (multi-pass membrane protein).
• Tissue specificity: Expressed in brain (temporal, frontal, parietal lobes, hippocampus, cerebellum), kidney, lung, liver, heart, skeletal muscle, placenta.
• The protein is subject to alternative splicing, glycosylation, phosphorylation, and disulfide bond formation; genetic defects are associated with familial hypocalciuric hypercalcemia, neonatal severe hyperparathyroidism, and autosomal dominant hypocalcemia.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 211-310) maps to the extracellular domain of human CaSR, favoring detection of surface receptor populations in flow cytometry.
• Validate antibody specificity in the relevant sample system (e.g., parathyroid or kidney cell lines) using isotype controls and antigen-blocking experiments.
• For optimal FC performance, titrate the antibody to determine the lowest concentration yielding maximum signal-to-noise ratio.
• Consider permeabilization protocols if intracellular staining is required; note that the epitope may be sensitive to fixation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, supplying this anti-CaSR monoclonal antibody alongside validated antibody pairs, optimized clones, and ancillary reagents. Our platform supports researchers and diagnostic developers in calcium-sensing receptor studies and related assay construction. Contact us for bulk quantities or customized sourcing solutions.