PE Rabbit Anti-Human Calreticulin Monoclonal Antibody for Intracellular Flow Cytometry - P27797

Core Product Specifications and Parameters

Biological Background: Calreticulin Function and Localization

• Calreticulin (CALR, also known as CRP55, Calregulin, ERp60, HACBP, grp60) is a highly conserved calcium-binding chaperone primarily localized to the endoplasmic reticulum (ER) lumen.
• It serves as a central component of the calreticulin/calnexin cycle, promoting the folding, oligomeric assembly, and quality control of newly synthesized glycoproteins by binding to monoglucosylated N-linked glycans (PubMed:7876246). Related references: PMID:7876246
• Calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor (NR3C1) and mediates its nuclear export, thereby linking ER function to cytoplasmic signaling pathways (PubMed:11149926). Related references: PMID:11149926
• Beyond the ER, calreticulin localizes to the cytosol, cell surface, extracellular matrix, and secretory vesicles; it is also detected in the sarcoplasmic reticulum, cortical granules, and cytolytic granules, reflecting its multifunctional roles.
• It participates in calcium homeostasis, cellular adhesion, and gene expression regulation, and has been implicated in oocyte maturation and the block to polyspermy.
• Post-translational modifications include acetylation, hydroxylation, and a conserved disulfide bond; the protein has a molecular weight of approximately 48 kDa and contains a signal peptide, lectin-binding domains, and metal-binding sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 335–417) of human calreticulin; verify that this epitope is accessible in your specific fixation and permeabilization protocol for intracellular flow cytometry.
• Since this antibody is PE-conjugated and recommended for intracellular staining, consider optimizing permeabilization conditions and including appropriate isotype controls to account for potential background due to intracellular Fc receptors or non-specific binding.
• Calreticulin is expressed in multiple subcellular compartments; validate staining specificity by comparing localization patterns in the ER, cytosol, or cell surface, depending on your experimental context.
• For applications other than intracellular flow cytometry, pilot testing is strongly recommended, as this antibody is validated only for the stated application under the supplier’s conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined path from concept to clinic by providing one-stop access to a comprehensive portfolio of IVD raw materials, technical services, and consultancy. This PE-conjugated anti-Calreticulin monoclonal antibody addresses the need for reliable immunodetection of a key ER chaperone implicated in protein folding, calcium signaling, and cancer-associated mutations. Through supply solutions that include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing capabilities, CamelBio supports the development of robust diagnostic assays and research tools.