PE Rabbit anti-Human C5AR1/CD88 Monoclonal Antibody for FC - P21730

Core Product Specifications and Parameters

Biological Background: C5AR1 Function and Localization

• C5AR1 (C5a anaphylatoxin chemotactic receptor 1, also known as C5a-R, C5aR, CD88) is a G protein-coupled receptor that serves as the receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a.
• Upon C5a binding, C5AR1 undergoes a conformational change that triggers signaling via guanine nucleotide-binding proteins (G proteins), primarily coupled to G(i)/G(o) alpha subunits (GNAI1 or GNAO1), leading to inhibition of adenylate cyclase. Related references: PMID:36806352, PMID:37852260
• Activation of C5AR1 stimulates a range of cellular responses including chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production, implicating it in inflammatory processes. Related references: PMID:10636859, PMID:15153520, PMID:1847994
• The ligand C5a interacts with at least two sites on C5AR1: a high-affinity site on the extracellular N-terminus and a second site within the transmembrane region that activates downstream signaling. Related references: PMID:7622471, PMID:8182049, PMID:9553099
• Subcellularly, C5AR1 localizes to the cell membrane and cytoplasmic vesicles, consistent with its roles in signal transduction and receptor trafficking.
• C5AR1 is post-translationally modified by glycosylation, phosphorylation, and sulfation, and contains disulfide bonds characteristic of GPCRs, as indicated by UniProt keywords.
• As a member of the G protein-coupled receptor family, C5AR1 functions as a transducer in chemotactic signaling pathways and is identified in proteomic studies.

Experimental Guidance and Technical Tips

• This antibody was generated against a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human C5AR1. For optimal performance in flow cytometry (FC), consider titrating the antibody to determine the appropriate working concentration for your specific cell type and experimental conditions.
• Due to the receptor's expression on cell membranes and intracellular vesicles, permeabilization steps may affect staining patterns; evaluate both surface and total staining protocols as needed.
• Validate the antibody specificity using a relevant positive control cell line known to express C5AR1 (e.g., immune cells) and a negative control (e.g., receptor-negative cells or isotype control).
• If using this PE-conjugated antibody in multicolor flow panels, ensure proper compensation and avoid potential spectral overlap with other fluorophores.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This PE-conjugated anti-human C5AR1/CD88 monoclonal antibody supports diagnostic manufacturers and researchers focused on inflammation, immunology, and GPCR signaling pathways. Whether you need custom conjugations, large-scale antibody supply, or complementary reagents, CamelBio streamlines your procurement from concept to clinic.