PE Rabbit anti-Chicken CD25 Monoclonal Antibody for FC - Q90WJ4

Core Product Specifications and Parameters

Biological Background: Interleukin-2 receptor subunit alpha (CD25) Function and Localization

• CD25 is the alpha subunit of the interleukin-2 (IL-2) receptor, essential for forming the high-affinity IL-2 receptor complex.
• It plays a central role in maintaining immune tolerance by regulating the activity of regulatory T cells (TREGs).
• TREGs expressing CD25 suppress the activation and expansion of autoreactive T cells, thereby preventing autoimmunity.
• The protein is a type I transmembrane glycoprotein located on the cell surface (Membrane), with a signal peptide and a single transmembrane helix.
• Structural features include sushi domains (repeat regions) and disulfide bonds that contribute to its receptor function.
• Known aliases include Il2r, Ly-43, p55, IL2R, TCGFR, and IDDM10, reflecting its association with immune regulation and disease.
• Post-translational modifications include glycosylation and disulfide bond formation, as indicated by the keywords Glycoprotein and Disulfide bond.

Experimental Guidance and Technical Tips

• This antibody is PE-conjugated and recommended for flow cytometry (FC). Direct staining protocols should be optimized for chicken cell populations.
• The immunogen encompasses the extracellular region (amino acids 21–190) of chicken CD25, suggesting the antibody binds to the accessible cell-surface domain.
• Use appropriate isotype controls and titrate the antibody to achieve optimal signal-to-noise ratios in FC experiments.
• For applications beyond FC, consider conducting validation experiments in the relevant sample system before use.
• Store at recommended temperatures, avoid repeated freeze-thaw cycles, and protect from light to preserve PE fluorescence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop sourcing solution for IVD raw materials. This PE-conjugated anti-chicken CD25 monoclonal antibody supports immunology and Treg research, and CamelBio can supply validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your diagnostic development from concept to clinic.