PE Mouse anti-Human TCR Vδ2 Monoclonal Antibody for FC - P04234

Core Product Specifications and Parameters

Biological Background: CD3 Delta Chain Function and Localization

• The CD3D gene encodes the CD3 delta chain, a critical component of the T-cell receptor-CD3 complex on T-lymphocytes, playing an essential role in adaptive immune responses.
• CD3 delta, together with CD3E, CD3G, and CD247, transmits activation signals across the plasma membrane after TCR engagement by peptide-MHC complexes on antigen-presenting cells.
• The cytoplasmic domain contains immunoreceptor tyrosine-based activation motifs (ITAMs) that are phosphorylated by Src kinases LCK and FYN, initiating downstream signaling cascades.
  Related references: PMID:2470098
• CD3D is indispensable for thymocyte differentiation; it ensures proper TCR-CD3 complex assembly and surface expression, and physically links the TCR to CD4 or CD8 co-receptors, a process required for positive selection of T-cells.
  Related references: PMID:12215456
• Subcellularly, the CD3 delta chain localizes predominantly to the cell membrane as a single-pass type I membrane protein.
• It is primarily expressed on T-lymphocytes as part of the mature TCR-CD3 complex and is also detected in fetal natural killer (NK) cells.
• Post-translational modifications include glycosylation and phosphorylation; the protein features a signal peptide, an extracellular immunoglobulin-like domain, a transmembrane helix, and a cytoplasmic ITAM-containing region.

Experimental Guidance and Technical Tips

• Since the immunogen is recombinant TCR Vδ2, verify that the antibody recognizes the native conformation on live cell surfaces during flow cytometry staining.
• Titrate the antibody to determine the optimal working concentration for your specific cell type and experimental conditions.
• Include proper isotype controls and, where possible, fluorescence-minus-one (FMO) controls to set accurate gating boundaries in multicolor panels.
• As a PE-conjugated reagent, handle and store the antibody protected from light to prevent photobleaching and signal loss.
• The reported cross reactivity is human; if testing non-human primate or other species, perform an independent validation.
• For consistent results, standardize fixation and permeabilization steps if intracellular staining is attempted, although the antibody is intended for surface staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical services for diagnostic and research applications, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. If your project involves T-cell immunophenotyping or gamma/delta T cell biology, we supply a range of fluorescently labeled antibodies against TCR chains and CD3 components to meet your assay requirements. Contact us to streamline your sourcing and accelerate development from concept to clinic.