PE Mouse anti-Human CD56/NCAM1 Monoclonal Antibody for Flow Cytometry - P13591

Core Product Specifications and Parameters

Biological Background: CD56/NCAM1 Function and Localization

• Neural cell adhesion molecule 1 (NCAM1), commonly known as CD56, belongs to the immunoglobulin superfamily and mediates homophilic and heterophilic cell–cell adhesion.
• NCAM1 plays a pivotal role in neuron–neuron adhesion, neurite fasciculation, and neurite outgrowth, contributing to neural development and plasticity.
• It serves as a host cell receptor for viruses such as rabies virus and Zika virus, facilitating their entry into cells.
• The protein is localized predominantly at the cell membrane, with secreted isoforms also present, and exists in multiple forms via alternative splicing, including transmembrane and GPI‑anchored variants.
• NCAM1 undergoes post‑translational modifications, including glycosylation and phosphorylation, which regulate its adhesive functions and signaling capabilities.
• Structurally, it contains multiple immunoglobulin‑like domains and fibronectin type III repeats, allowing for diverse molecular interactions.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls to assess non‑specific binding and ensure the specificity of PE staining.
• CD56 is expressed on NK cells, subsets of T cells, and neural tissues; validate the staining pattern in a relevant human sample system.
• Although the immunogen is a recombinant protein, the antibody may detect native protein; confirm performance if using in other applications like immunofluorescence or Western blotting after validation.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with streamlined access to high‑quality IVD raw materials, including validated monoclonal antibodies, bulk ancillary reagents, and technical support. Our portfolio of NCAM1 antibodies aids neurobiology and infectious disease research. For tailored sourcing solutions and one‑stop support from concept to clinic, contact our team.