PE Mouse anti-Human CD45RO Monoclonal Antibody for Flow Cytometry - P08575

Core Product Specifications and Parameters

Biological Background: CD45RO Function and Localization

• CD45, officially designated Receptor-type tyrosine-protein phosphatase C (PTPRC), is a transmembrane glycoprotein also known as Leukocyte common antigen (L-CA) and T200.
• It functions as a critical tyrosine phosphatase required for T-cell activation upon antigen receptor stimulation, dephosphorylating key signaling molecules such as LYN, SKAP1, and FYN (PubMed:35767951). Related references: PMID:35767951
• CD45 interacts with CLEC10A on immature dendritic cells at the immunological synapse to modulate T-cell activation thresholds and limit autoreactivity.
• The protein acts as a receptor for human cytomegalovirus protein UL11, mediating viral binding to T-cells and impairing T-cell receptor signaling and proliferation.
• Multiple isoforms are generated by alternative splicing, with CD45RO being a predominant isoform expressed on memory and activated T-cells (isoform 5 in thymocytes per UniProt tissue specificity).
• Subcellularly, CD45 localizes to the cell membrane, membrane rafts, and regulates signaling at the immunological synapse.
• The protein undergoes extensive post-translational modifications including glycosylation and phosphorylation, as indicated by keywords.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to PE, eliminating the need for secondary staining in flow cytometry.
• Consider using appropriate isotype controls and Fc receptor blocking for human samples to ensure specificity.
• Optimize the antibody concentration by titration in your specific cell system, as CD45RO expression can vary across leukocyte subsets.
• For intracellular staining, note that CD45RO is a cell surface marker; ensure membrane permeabilization if combined with intracellular detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering this anti-CD45RO monoclonal antibody alongside validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing. With expertise in immunology and T-cell biology, we support diagnostic manufacturers and research labs in developing high-quality assays for immune monitoring and disease diagnosis.