PE Mouse anti-Human CD42a Monoclonal Antibody for FC - P14770

Core Product Specifications and Parameters

Biological Background: GP-IX (CD42a) Function and Localization

• Platelet glycoprotein IX (GP-IX), also designated CD42a, is a 19-kDa membrane-bound subunit of the GPIb-V-IX receptor complex.
• Together with GPIbα, GPIbβ, and GPV, GP-IX forms the von Willebrand factor (vWF) receptor complex, which is essential for platelet adhesion to damaged vessel walls.
• The GPIb-V-IX complex mediates initial platelet tethering and activation at sites of vascular injury, a critical step in hemostasis and arterial thrombosis.
• GP-IX facilitates proper membrane insertion and spatial orientation of the GPIb subunits within the complex.
• The protein contains leucine-rich repeat (LRR) domains, which are important for protein-protein interactions and receptor stability.
• Mutations in the GP9 gene are associated with Bernard-Soulier syndrome, a rare bleeding disorder characterized by giant platelets and defective platelet adhesion.
• Subcellular localization is restricted to the platelet plasma membrane, consistent with its role in surface receptor signaling.
• Keywords: Blood coagulation, cell adhesion, hemostasis, leucine-rich repeat, membrane, signal, transmembrane, disease variant.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the PE-conjugated antibody to determine the optimal staining concentration in your specific platelet preparation.
• Use fresh, washed platelets or platelet-rich plasma; avoid platelet activation during sample handling to minimize background.
• Include appropriate isotype control antibodies conjugated to PE to assess non-specific binding.
• Fixation with paraformaldehyde after staining may preserve fluorescence but can alter scatter properties; validate in your system.
• CD42a expression is platelet‑specific; confirm gating using forward and side scatter or double staining with other platelet markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with high-quality raw materials for hemostasis and platelet biology applications. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare targets upon request. Contact us to streamline your procurement and accelerate assay development.