PE Mouse anti-Human CD1a Monoclonal Antibody for FC - P06126

Core Product Specifications and Parameters

Biological Background: CD1a Function and Localization

• CD1a is a T-cell surface glycoprotein, also known as T6/Leu-6, encoded by the CD1A gene (GeneID: 909).
• It belongs to the CD1 family of antigen-presenting molecules that bind self and non-self lipid and glycolipid antigens.
• CD1a presents lipid antigens to T-cell receptors on natural killer T (NKT) cells, playing a key role in innate-like immunity.
• The protein is localized to the cell membrane, membrane rafts, and endosomal membranes, facilitating antigen loading in endosomal compartments.
• CD1a is expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, and certain T-cell leukemias, with broader tissue distribution.
• It contains disulfide bonds, glycosylation sites, and a transmembrane helix, typical of immunoglobulin superfamily members.
• Research applications include studying NKT cell activation, lipid antigen presentation in infection and cancer, and screening of self-lipid-reactive T cells.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic peptide, confirm specificity by testing on CD1a-positive (e.g., MOLT-4) and CD1a-negative cell lines before experimental use.
• For flow cytometry, titrate the antibody to determine optimal staining concentration in the relevant sample system (e.g., peripheral blood mononuclear cells).
• Include appropriate isotype controls and viability staining to gate live CD1a-positive populations.
• If using for functional studies, consider that PE conjugation may affect antibody affinity; validate in your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated one-stop partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This PE anti-human CD1a monoclonal antibody supports immunology diagnostic development, and we can help source additional reagents for lipid antigen presentation research or supply large-scale production lots.