PE Mouse anti-Human CD15/SSEA-1 Monoclonal Antibody for FC - P22083

Core Product Specifications and Parameters

Biological Background: CD15/SSEA-1 Function and Localization

• FUT4 (fucosyltransferase 4) is a member of the glycosyltransferase family, responsible for transferring fucose to N-acetylglucosamine on glycoproteins, thereby generating the Lewis X (CD15) carbohydrate antigen.
• Catalyzes the alpha(1→3) linkage of fucose to distal N-acetylglucosamine of type 2 lactosamine chains, robustly forming Lewis X (CD15), and with lower efficiency, sialyl Lewis X and 6-sulfo sialyl Lewis X—key selectin recognition determinants (PubMed:1702034, PubMed:1716630, PubMed:29593094). Related references: PMID:1702034 PMID:1716630 PMID:29593094
• Together with FUT7, contributes to the biosynthesis of selectin ligands (e.g., SELE, SELL, SELP), mediating lymphocyte homing, leukocyte extravasation, and immune cell homeostasis (By similarity).
• In certain cell types, it may fucosylate internal LacNAc units to form the VIM-2 antigen, a SELE (E-selectin) epitope (PubMed:11278338, PubMed:1716630). Related references: PMID:11278338 PMID:1716630
• Subcellularly, the enzyme resides in the Golgi apparatus membrane, where it performs its catalytic function.
• Tissue expression is highest in hematopoietic cells—found in bone marrow mesenchymal stem cells, cord blood promyelocytes, and peripheral myeloid and lymphoid lineages—consistent with a role in leukocyte trafficking and inflammation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from human CD15/SSEA-1; the antibody likely recognizes the Lewis X glycan epitope; performance should be validated in specimens expressing endogenous FUT4.
• For flow cytometry (FC), titrate the antibody to determine the optimal staining concentration for your cell type; inclusion of appropriate isotype controls and Fc receptor blocking is recommended.
• PE conjugation provides high sensitivity; avoid repeated freeze-thaw cycles, and protect from light during storage and handling.
• Confirm CD15 expression in your sample using known positive controls (e.g., granulocytes) and negative controls (e.g., lymphoid cells that do not express CD15).
• Note that CD15/SSEA-1 is expressed on both myeloid cells and some stem cells; thus, experimental design should consider target cell identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including this PE-conjugated anti-CD15/SSEA-1 monoclonal antibody, to support diagnostic and research applications in immunophenotyping, leukocyte analysis, and stem cell characterization. Our portfolio encompasses validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, offering a streamlined procurement experience from concept to clinic. Partner with us to accelerate your assay development with reliable, high-quality reagents.