PE Mouse anti-Human CD11c Monoclonal Antibody for Flow Cytometry - P20702

Core Product Specifications and Parameters

Biological Background: Integrin alpha-X (CD11c) Function and Localization

• Integrin alpha-X (CD11c, ITGAX) is a leukocyte adhesion glycoprotein also known as Leu M5 and CD11 antigen-like family member C, belonging to the integrin alpha chain family.
• It pairs with the beta-2 subunit (CD18) to form the heterodimeric receptor integrin alpha-X/beta-2 (p150,95), which functions as a fibrinogen receptor by recognizing the G-P-R sequence, mediating cell-cell interactions during inflammatory responses.
• The receptor is critically involved in monocyte adhesion and chemotaxis, facilitating leukocyte migration and immune surveillance.
• ITGAX also serves as a receptor for the erythrocyte-specific intercellular adhesion molecule ICAM4, contributing to erythrophagocytosis. Related references: PMID:16985175
• Subcellularly, CD11c is localized at the cell membrane as a single-pass type I membrane glycoprotein.
• Its expression is predominantly restricted to monocytes and granulocytes, consistent with its role in innate immunity.
• The integrin alpha-X protein requires divalent cations (calcium and magnesium) for its adhesive functions and contains metal-binding domains characteristic of integrins.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is suitable for flow cytometry analysis of CD11c expression on human leukocytes, particularly monocytes and granulocytes.
• Consider titrating the antibody to determine the optimal staining index for your specific experimental setup.
• Since the immunogen is recombinant full-length human CD11c, it may recognize native conformation on cell surfaces; validate in the relevant sample system.
• If using for other applications such as immunohistochemistry or western blotting, perform in-house validation due to potential conformational sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with seamless access to IVD raw materials, technical services, and consulting from concept to clinic. This PE-conjugated anti-human CD11c monoclonal antibody supports flow cytometry-based immunophenotyping applications, and CamelBio offers complementary sourcing of validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For rare or custom targets like integrin family members, our dedicated supply chain ensures reliable procurement.