PE Mouse anti-Human CD11b Monoclonal Antibody for FC - P11215

Core Product Specifications and Parameters

Biological Background: CD11b Function and Localization

• Integrin alpha-M (ITGAM/CD11b) is a leukocyte integrin alpha chain that pairs with integrin beta-2 (ITGB2/CD18) to form the Mac-1 (CR-3) complex.
• ITGAM/ITGB2 mediates adhesive interactions of monocytes, macrophages, and granulocytes and serves as the CR-3 receptor for the iC3b fragment of complement component C3, promoting uptake of complement-coated particles and pathogens. Related references: PMID:20008295, PMID:9558116
• ITGAM/ITGB2 acts as a receptor for fibrinogen, factor X, and ICAM1; ICAM1 binding facilitates adhesion between stimulated neutrophils and endothelial cells. Related references: PMID:1980124
• The complex is required for CD177-PRTN3-mediated activation of TNF-primed neutrophils and regulates neutrophil migration. Related references: PMID:21193407, PMID:28807980
• Predominantly expressed in monocytes and granulocytes, with protein expression confirmed in neutrophils; localizes to the cell membrane and membrane rafts. Related references: PMID:1346576, PMID:21193407
• Structural features include a signal peptide, multiple extracellular integrin alpha repeats, a transmembrane helix, disulfide bonds, and N-glycosylation sites.
• Beyond adhesion, ITGAM/ITGB2 may contribute to phagocytosis-induced apoptosis in extravasated neutrophils, mast cell development, and, in microglia, TYROBP/DAP12-dependent superoxide production that promotes neuronal apoptosis during brain development.
• Genetic variants in ITGAM are associated with systemic lupus erythematosus (SLE), underscoring its role in immune regulation.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• As the immunogen is a synthetic peptide, the epitope is likely linear; the antibody may be compatible with both live cells and fixed/permeabilized protocols, but validation in your system is recommended.
• Use appropriate compensation controls (e.g., single-stained cells or compensation beads) to correct for spectral overlap of PE with other fluorophores.
• Although specificity is reported for human CD11b, cross-reactivity with other species should be verified if needed; consider sequence alignment of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, supporting research and diagnostic development in innate immunity and inflammation. This anti-CD11b monoclonal antibody exemplifies our portfolio of validated flow cytometry reagents, complemented by antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. With an integrated supply chain, we help manufacturers accelerate assay development from concept to clinic.