PE-conjugated Anti-Mouse CD22 Monoclonal Antibody for Flow Cytometry - P35329

Core Product Specifications and Parameters

Biological Background: CD22 Function and Localization

• CD22 (Siglec-2, BL-CAM) is a sialic acid-binding immunoglobulin-like lectin predominantly expressed on B-cells, with alternative splicing yielding multiple isoforms (predicted molecular weights 89–97 kDa).
• It plays essential roles in B-cell biology, including differentiation, antigen presentation, and bone marrow trafficking. Related references: PMID:7533044
  PMID:9016707
• CD22 binds α2,6-linked sialic acids on cis ligands (e.g., CD22, CD45, IgM) and trans ligands on opposing cells, mediating cell adhesion.
• As an inhibitory coreceptor, it dampens BCR signaling: upon LYN-mediated ITIM phosphorylation, it recruits SHP-1 phosphatase, inhibiting calcium mobilization and cell activation. Related references: PMID:9016707
• Subcellularly, CD22 is localized to the plasma membrane of B-lymphocytes.
• The protein features immunoglobulin domains, disulfide bonds, and regulatory phosphorylation sites.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 22-151) of mouse CD22, which may recognize both cis- and trans-interacting forms; validate specificity in appropriate model systems.
• For flow cytometry, titrate the antibody to determine optimal staining concentration; include appropriate isotype controls and consider Fc receptor blocking.
• As a PE-conjugated antibody, ensure proper compensation when using multicolor panels; protect from light to prevent fluorophore bleaching.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This PE-conjugated anti-CD22 antibody supports B-cell immunophenotyping and leukemia/lymphoma research applications. Our sourcing network can also assist with rare target raw-material procurement and custom development to accelerate your IVD assay pipeline.