PE-CF594 Rabbit anti-Human/Mouse PDGFRα/CD140a Monoclonal Antibody for FC - P16234

Core Product Specifications and Parameters

Biological Background: PDGFRα Function and Localization

• PDGFRα (CD140a, PDGFR2) is a cell-surface tyrosine-protein kinase receptor that binds platelet-derived growth factors PDGFA, PDGFB, and PDGFC, playing an essential role in embryonic development, cell proliferation, survival, and chemotaxis.
• It is critical for normal skeleton development and cephalic closure during embryogenesis, and for differentiation of bone marrow-derived mesenchymal stem cells.
• Upon ligand binding, PDGFRα activates multiple signaling cascades, including PI3K-AKT, RAS-MAPK, and STAT pathways; it phosphorylates key substrates such as PIK3R1, PLCG1, and PTPN11.
• The receptor is involved in wound healing by promoting cell migration and chemotaxis, and contributes to platelet activation, agonist secretion, and thrombin-induced aggregation.
• PDGFRα localizes to the cell membrane, cell projections, cilia, and the Golgi apparatus.
• It is widely expressed, with detection in platelets, brain, fibroblasts, smooth muscle, heart, embryo, and primary and metastatic colon tumors.
• As a proto-oncogene and disease-associated glycoprotein, PDGFRα features immunoglobulin-like domains, disulfide bonds, alternative splicing, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• This antibody is conjugated to PE-CF594, a bright fluorophore well-suited for multicolor flow cytometry panels; consider titrating the antibody to optimize staining resolution and minimize background.
• The immunogen corresponds to the extracellular domain (amino acids 24–524), suggesting recognition of the receptor's extracellular region; surface staining of live cells is expected, but include appropriate isotype and fluorescence-minus-one controls.
• Because PDGFRα is expressed on diverse cell types, validate expression in your specific sample system using known positive and negative controls.
• For intracellular detection, evaluate fixation and permeabilization protocols, as these may affect epitope accessibility and fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop partner for diagnostic manufacturers and research institutions, supplying a comprehensive range of IVD raw materials—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. This PE-CF594 anti-PDGFRα monoclonal antibody supports oncology, developmental biology, and platelet research applications. Let CamelBio streamline your procurement for reliable, well-characterized reagents tailored to your assay development needs.