PE-CF594 Rabbit anti-Human/Monkey CD69 mAb for Flow Cytometry - Q07108

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• CD69 (Cluster of Differentiation 69), also known as activation inducer molecule (AIM), EA1, or CLEC2C, is a transmembrane C-type lectin protein encoded by the CD69 gene in humans.
• It is rapidly expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells, and platelets, playing a crucial role in immune cell homeostasis.
• CD69 negatively regulates Th17 cell differentiation via its carbohydrate-dependent interaction with galectin-1 (LGALS1) on immature dendritic cells, involving the JAK3/STAT5 signaling pathway. Related references: PMID:24752896
• It promotes the conversion of naive CD4 T-cells into regulatory T-cells through interaction with the S100A8/S100A9 complex on peripheral blood mononuclear cells. Related references: PMID:26296369
• CD69 acts as an oxidized low-density lipoprotein (oxLDL) receptor in CD4 T-lymphocytes, negatively regulating inflammatory responses by inducing PDCD1 expression via NFAT activation. Related references: PMID:35930205
• In the context of antibacterial defense, CD69 recognizes Pseudomonas aeruginosa and triggers ERK1 activation, leading to the secretion of GM-CSF and other inflammatory cytokines. Related references: PMID:34841721
• CD69 negatively regulates the chemotactic responses of effector lymphocytes and dendritic cells by acting as an S1PR1 agonist, facilitating receptor internalization and degradation. Related references: PMID:37039481
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation; the protein localizes to the cell membrane as a type II transmembrane protein.

Experimental Guidance and Technical Tips

• This PE-CF594-conjugated antibody is ready-to-use for flow cytometry; titrate to determine the optimal working concentration for your cell type and staining protocol.
• Include appropriate isotype control and viability dye to accurately gate CD69-positive populations, especially when analyzing rare activated subsets.
• CD69 is rapidly upregulated upon in vitro stimulation; consider time-course experiments to capture peak expression (e.g., 4–6 hours post-PMA/ionomycin activation for T-cells).
• Cross-reactivity with cynomolgus monkey CD69 has been demonstrated; validate in your non-human primate model to confirm reactivity.
• When designing multicolor panels, account for PE-CF594’s excitation and emission spectra (optimally excited by 488 nm or 561 nm lasers) and perform compensation using single-stained controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This CD69 antibody supports immunophenotyping and immune activation research in flow cytometry applications. Our team specializes in sourcing rare and custom targets, ensuring reliable supply chains from concept to clinic.