PE-CF594 Rabbit anti-Human BCL6 mAb - P41182

Core Product Specifications and Parameters

Biological Background: BCL6 Function and Localization

• BCL6 is a transcriptional repressor primarily required for germinal center formation and antibody affinity maturation.
• It binds directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) and forms complexes with corepressors and histone deacetylases to repress target genes.
• In germinal center B-cells, BCL6 represses genes involved in differentiation, inflammation, apoptosis, and cell cycle control; it also indirectly upregulates AICDA through microRNA repression.
• BCL6 promotes follicular helper T-cell gene expression while inhibiting differentiation of Th1, Th2, and Th17 cells, and is essential for immunological memory in both T- and B-cells.
• The protein localizes to the nucleus and is highly expressed in germinal center T- and B-cells, as well as in primary immature dendritic cells.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination; it features zinc-finger domains and is classified as a proto-oncogene.
• Additionally, BCL6 regulates neurogenesis by altering NOTCH-dependent transcriptional complexes and recruiting SIRT1 to silence selective NOTCH targets.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fragment (aa 235-350) of human BCL6, which includes C-terminal zinc finger regions; consider verifying reactivity with full-length protein in your experimental system.
• For intracellular flow cytometry, optimize fixation and permeabilization steps suitable for nuclear antigens; titrate the antibody to determine the optimal working concentration.
• Use appropriate negative controls (e.g., BCL6-deficient cell lines or isotype control) and validate staining specificity under your assay conditions.
• This antibody is validated for intracellular flow cytometry with human samples; other applications have not been tested and may require user validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering monoclonal antibodies, recombinant proteins, and technical support for immunology and hematology research. Our product portfolio covers BCL6 and other germinal center markers, enabling streamlined development of diagnostic assays and research tools. Leverage our sourcing capabilities for rare-target reagents and bulk ancillary materials to take your project from concept to clinic.