PE Anti-VEGFR-3/FLT-4 Rabbit Monoclonal Antibody for Flow Cytometry - P35916

Core Product Specifications and Parameters

Biological Background: VEGFR-3/FLT-4 Function and Localization

• VEGFR-3, also known as FLT-4, is a receptor tyrosine kinase that binds VEGFC and VEGFD, playing a central role in lymphangiogenesis and early cardiovascular development.
• It promotes endothelial cell proliferation, survival, and migration, and regulates angiogenic sprouting.
• Activated VEGFR-3 triggers downstream signaling via MAPK/ERK, JUN, and AKT1 pathways, and phosphorylates SHC1 and PIK3R1.
• A secreted isoform (isoform 3) may act as a decoy receptor, negatively regulating VEGFC-mediated lymphangiogenesis.
• The protein is located at the cell membrane, but also detected in cytoplasm and nucleus; alternative splicing generates isoforms with distinct C-termini and signaling efficiencies.
• Widely expressed in adult tissues (liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, kidney) and detected in endothelial cells.
• Post-translational modifications include phosphorylation and glycosylation; categorized as a disulfide-linked glycoprotein with immunoglobulin-like domains.
• Mutations in FLT4 are associated with hereditary lymphedema (e.g., Milroy disease), highlighting its clinical significance.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine optimal staining concentration. Include appropriate isotype and compensation controls for PE.
• The immunogen corresponds to the extracellular domain (aa 25-776); therefore, this antibody should bind surface-expressed VEGFR-3 on live cells.
• Verify expression in the target cell type (e.g., human lymphatic endothelial cells) using known positive controls before experimental use.
• Ensure proper handling and storage at 2–8°C protected from light to maintain conjugate stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop partner for IVD raw materials, providing diagnostic manufacturers, labs, and research institutes with reliable access to critical reagents like this PE-conjugated anti-VEGFR-3 monoclonal antibody for flow cytometry. Our sourcing capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom procurement of rare targets, supporting your projects from concept to clinic.