PE Anti-Mouse/Human CD55 Monoclonal Antibody for Flow Cytometry - Q61475

Core Product Specifications and Parameters

Biological Background: CD55/DAF Function and Localization

• CD55, also known as decay-accelerating factor (DAF), is a GPI-anchored membrane glycoprotein that protects host cells from autologous complement attack.
• It recognizes C4b and C3b fragments deposited on cell surfaces and interferes with the assembly and stability of the classical and alternative pathway C3 and C5 convertases, thereby inhibiting the complement cascade.
• The protein contains multiple short consensus repeat (SCR) domains, characteristic of the regulators of complement activation (RCA) family.
• Subcellular localization is primarily at the cell membrane via a C-terminal GPI anchor, with delivery to the surface following signal peptide cleavage.
• Tissue expression is broad, including brain, secretory epithelia, skeletal muscle, liver, testes, thymus, spleen, and lymph nodes.
• Post-translational modifications include disulfide bonds, N-linked glycosylation, and addition of the GPI moiety.
• Keywords from UniProt include cell membrane, complement pathway, glycoprotein, GPI-anchor, innate immunity, and sushi domains, reflecting its role in immune regulation.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 35-361) spans the extracellular region of mouse CD55/DAF, which is likely to present native conformational epitopes on live cells.
• PE conjugation enables direct single-step staining for flow cytometry without the need for secondary detection reagents.
• Cross-reactivity with both human and mouse CD55 may facilitate translational studies; independent validation in the relevant sample system is advisable.
• For optimal resolution, consider using appropriate isotype controls and performing antibody titration in the intended cell type.
• Note that GPI-anchored proteins may be released from the cell surface under certain conditions; gentle handling of samples is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner for diagnostic manufacturers, labs, and research institutes. We support complement research and immunoassay development by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For CD55/DAF-related projects, CamelBio can facilitate procurement of rare-target raw materials, streamlining your transition from concept to clinic.