PE Anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody for Flow Cytometry - P18468 / P06342 / Q3U060 / P14435

Core Product Specifications and Parameters

Biological Background: MHC Class II (I-A/I-E) Function and Localization

• The I-A and I-E beta chains are core components of mouse major histocompatibility complex (MHC) class II molecules, encoded by genes such as H2-Ab1 and H2-Eb1, respectively. These proteins are pivotal for adaptive immunity by presenting antigenic peptides to CD4+ T helper cells.
• Each MHC class II heterodimer consists of an alpha chain and a beta chain; this antibody recognizes beta chains from both I-A and I-E isotypes, enabling broad detection of MHC class II expression.
• Subcellularly, MHC class II beta chains are single‑pass type I membrane proteins that cycle between the plasma membrane and endosomal compartments, where peptide loading occurs before trafficking to the cell surface.
• Key post‑translational modifications include disulfide bond formation in the extracellular domain, N‑linked glycosylation, and ubiquitination, which regulate protein stability and MHC II turnover.
• The molecules are classified within the immunoglobulin superfamily and possess a characteristic structure: a signal peptide, two extracellular domains (α1/β1 and α2/β2), a transmembrane helix, and a short cytoplasmic tail.
• As central mediators of the adaptive immune synapse, MHC class II molecules bridge the innate and adaptive systems, ensuring specific T‑cell activation and immune surveillance.
• Tissue expression is predominantly on professional antigen‑presenting cells (dendritic cells, macrophages, B cells) and thymic epithelial cells, but can be induced on other cell types by inflammatory stimuli.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein encompassing I-A/I-E sequences; for similar reagent design, full‑length or extracellular domain constructs may help preserve native conformation recognition.
• This PE‑conjugated antibody is intended for flow cytometry. Titrate the antibody in your relevant cell system, and include a proper isotype control and Fc receptor blocking step to minimize background.
• The antibody exhibits mouse cross‑reactivity; validation is recommended if samples from other rodent species are tested.
• Predicted molecular weights of target beta chains range from 26 kDa to 32 kDa. Note that the antibody has not been validated for denaturing western blotting; if attempted, optimization under native or non‑reducing conditions may be required.
• Because the antibody recognizes multiple beta chain variants, it serves as a pan‑MHC class II tool. Verify expression patterns in the specific mouse strain or cell line to be used.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to high‑quality IVD raw materials, including validated monoclonal antibodies such as this anti‑mouse MHC Class II conjugate. We supply optimized antibody pairs, bulk ancillary reagents, and facilitate sourcing of rare‑target raw materials, streamlining your transition from concept to clinic. Partner with CamelBio as your single‑source bridge for reliable, production‑grade immunological reagents and dedicated technical guidance.