PE Anti-Mouse CD69 Monoclonal Antibody for Flow Cytometry - P37217

Core Product Specifications and Parameters

Biological Background: CD69 Function and Localization

• Transmembrane protein expressed mainly on mucosal T‑cells, essential for immune cell homeostasis; rapidly upregulated on platelets, T‑lymphocytes, and NK cells upon activation, stimulating different signaling pathways. Related references: PMID:20696842, PMID:38540778
• Negatively regulates Th17 cell differentiation through carbohydrate‑dependent interaction with galectin‑1/LGALS1 on immature dendritic cells. Association with the JAK3/STAT5 signaling pathway controls RORγt transcription. Related references: PMID:20696842
• Promotes conversion of naive CD4 T‑cells into regulatory T‑cells via the S100A8/S100A9 complex; acts as an oxidized LDL receptor, negatively regulating inflammation by inducing PDCD1 expression through NFAT activation.
• Participates in adipose tissue‑derived mesenchymal stem cell‑mediated protection against P. aeruginosa infection by recognizing the bacteria and promoting ERK1 activation and GM‑CSF secretion.
• In eosinophils, induces IL‑10 production through the ERK1/2 pathway. Related references: PMID:38540778
• Negatively regulates chemotactic responses to sphingosine‑1‑phosphate by acting as a S1PR1 agonist and facilitating receptor internalization and degradation. Related references: PMID:16525420
• Expressed on the surface of activated T‑cells, B‑cells, NK cells, neutrophils, platelets, and eosinophils. Related references: PMID:38540778
• Subcellular localization: Cell membrane

Experimental Guidance and Technical Tips

• The PE conjugate enables direct flow cytometric detection of CD69 on activated mouse leukocytes. Consider using fresh or appropriately stimulated cells for optimal surface staining.
• The immunogen (aa 64–199) covers the C‑type lectin‑like extracellular domain; this design supports recognition of native surface CD69, which is critical for flow cytometry.
• Validate staining in your specific experimental system (e.g., stimulated splenocytes, PBMCs, or cultured cell lines). Include appropriate isotype controls and viability dyes to exclude dead cells.
• If intracellular detection is attempted, optimize fixation and permeabilization conditions thoroughly, as the epitope may be sensitive to harsh treatments.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio is a dedicated one‑stop IVD raw‑material partner, supplying diagnostic manufacturers and research laboratories with validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents. With this PE anti‑mouse CD69 antibody, you gain access not only to a well‑characterized flow‑cytometry reagent for immunology and inflammation research, but also to CamelBio’s full portfolio of bulk‑sourcing options, technical consulting, and rare‑target raw‑material procurement. Whether advancing T‑cell activation studies or developing diagnostic assays, we bridge your concept to clinic with reliable supply solutions.