PE anti-Mouse CD4 Monoclonal Antibody for Flow Cytometry - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• CD4 is an integral membrane glycoprotein that serves as a coreceptor for MHC class II molecules, playing a critical role in T-cell activation and immune responses.
• It interacts simultaneously with the T-cell receptor (TCR) and MHC class II on antigen-presenting cells, recruiting the Src kinase LCK to initiate intracellular signaling cascades leading to T-cell proliferation, cytokine production, and differentiation.
• CD4 is a hallmark of T-helper cells, highly expressed on these cells, but also present on other immune cells such as macrophages, dendritic cells, and NK cells, where it can function in a TCR/LCK-independent manner.
• In the thymus, CD4 participates in the development of T-helper cells, and it can trigger the differentiation of monocytes into functional mature macrophages.
• The protein contains immunoglobulin-like domains, disulfide bonds, and is subject to post-translational modifications including N-linked glycosylation, palmitoylation, and phosphorylation.
• Alternative splicing results in multiple isoforms, and the protein is anchored in the cell membrane via a single-pass transmembrane domain.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is optimized for flow cytometry; consider titrating the antibody for your specific cell type and staining conditions to achieve optimal signal-to-noise ratio.
• CD4 is expressed on T-helper cells and subsets of other immune cells; validate staining patterns using appropriate positive (e.g., mouse splenocytes) and negative (e.g., CD4 knockout samples) controls.
• For multicolor flow cytometry panels, ensure compatibility of PE with other fluorophores and perform compensation as needed.
• The immunogen corresponds to the extracellular domain (aa 27-394), so the antibody likely recognizes an extracellular epitope, suitable for live cell staining without permeabilization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. Our portfolio includes validated monoclonal antibodies and antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. For CD4 and other immune cell markers, we offer a range of primary antibodies, conjugated formats, and matched pairs to support your immunology assay development and immune profiling needs.