PE anti-Mouse CD11c mAb - Q9QXH4

Core Product Specifications and Parameters

Biological Background: Integrin alpha-X Function and Localization

• Integrin alpha-X, also known as CD11c (CD11 antigen-like family member C), is a leukocyte adhesion glycoprotein that pairs with the integrin beta-2 chain to form the p150,95 heterodimer.
• It functions as a receptor for fibrinogen, recognizing the G-P-R sequence, and mediates cell-cell interactions during inflammatory responses.
• CD11c plays a crucial role in monocyte adhesion and chemotaxis, contributing to the recruitment of immune cells to sites of inflammation.
• Additionally, it serves as a receptor for the erythrocyte-specific adhesion molecule ICAM4, mediating erythrophagocytosis.
• The protein is located at the cell membrane, consistent with its role in cell adhesion and signaling.
• CD11c contains intrinsic calcium and magnesium binding sites and features disulfide bonds, which are characteristic of integrin alpha subunits.
• Keywords associated with CD11c include Calcium, Cell adhesion, Cell membrane, Disulfide bond, Glycoprotein, Integrin, Magnesium, Membrane, Metal-binding, Receptor, Repeat, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein of mouse CD11c, suggesting the antibody may recognize a native conformation; this property supports its application in flow cytometry (FC) for detecting surface CD11c on live cells.
• For flow cytometry, titrate the antibody to determine the optimal dilution for your specific cell type, and include appropriate isotype and fluorescence-minus-one (FMO) controls.
• As a PE-conjugated antibody, protect from light during staining and storage, and follow standard protocols for surface staining and data acquisition.
• Some loss of signal is possible if fixation and permeabilization are required; validate the compatibility of this antibody with your fixation method.
• Given that cross-reactivity is confirmed only for mouse, this antibody is suitable for mouse models; verify reactivity in your specific strain or biological context if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive B2B partner for IVD raw materials, and this PE anti-Mouse CD11c mAb exemplifies our offering of validated antibodies for critical research areas like immunology and cell adhesion. Our portfolio includes optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing services for rare targets. Whether advancing diagnostic assays or fundamental discovery, we bridge the gap from concept to clinic with reliable raw materials and expert support.