PE Anti-Human CD27 Monoclonal Antibody for Flow Cytometry - P26842

Core Product Specifications and Parameters

Biological Background: CD27 Function and Localization

• CD27 (also known as TNFRSF7) is a costimulatory immune-checkpoint receptor of the tumor necrosis factor receptor superfamily, expressed on T-cells, NK-cells, and B-cells.
• Binding to its ligand CD70/CD27L triggers CD27-mediated T-cell activation and expansion, which in turn facilitates immune surveillance of B-cells. Related references: PMID:28011863
• Mechanistically, CD70 ligation activates the TRAF2-PTPN6 signaling axis, leading to inhibition of LCK phosphorylation and promoting T-cell memory differentiation. Related references: PMID:38354704
• CD27 also negatively regulates Th2 lymphocyte function in adipose tissue and provides a negative feedback signal to leukocyte differentiation during immune activation.
• CD27 is a single-pass type I membrane protein localized to the cell membrane.
• The protein undergoes post-translational modifications including glycosylation and phosphorylation, and contains structural features such as a signal peptide, transmembrane helix, and disulfide bonds.
• CD27 is predominantly expressed in T-lymphocytes, making it a key marker for T-cell subset analysis.
• The molecular weight of CD27 is approximately 29 kDa (29137 Da according to UniProt).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 21-192 of human CD27 (NP_001233.2), which spans the extracellular domain, enabling recognition of cell surface CD27.
• For flow cytometry, optimal antibody dilution and staining conditions should be empirically determined using CD27-expressing cells, such as human peripheral blood mononuclear cells (PBMCs) or T-cell lines.
• Consider co-staining with T-cell lineage markers (e.g., CD3, CD4, CD8) to characterize CD27 expression on specific lymphocyte subsets.
• Use appropriate isotype controls and Fc receptor blocking to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive IVD raw material sourcing partner, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare target development. This PE-conjugated anti-human CD27 antibody supports immunology and immuno-oncology research by enabling detection of the CD27 immune-checkpoint receptor. With an integrated supply chain and technical expertise, CamelBio helps bridge your development needs from concept to clinic.