PE Anti-Human CD22 Monoclonal Antibody for Flow Cytometry - P20273

Core Product Specifications and Parameters

Biological Background: CD22 Function and Localization

• CD22 (Siglec-2, BL-CAM, Leu-14) is a sialic acid-binding immunoglobulin-like lectin predominantly expressed on B-lymphocytes, functioning as both an adhesion molecule and an inhibitory coreceptor of the B-cell receptor (BCR). Related references: PMID:34330755 PMID:8627166
• Through its immunoreceptor tyrosine-based inhibitory motif (ITIM), CD22 is phosphorylated by Src family kinase LYN, recruiting the tyrosine phosphatase PTPN6/SHP-1 to negatively regulate BCR-induced calcium mobilization and cellular activation. Related references: PMID:8627166
• CD22 mediates cell adhesion via trans interaction with alpha 2,6-linked sialic acid residues on other cells, contributing to B-cell trafficking and homing to bone marrow. Related references: PMID:20172905 PMID:34330755
• Strong negative signaling through CD22 can trigger B-cell apoptosis, highlighting its role in controlling B-cell survival. Related references: PMID:20516366
• CD22 is a single-pass type I membrane protein localized on the cell membrane, and it undergoes alternative splicing producing isoforms with distinct cytoplasmic domains.
• The protein is heavily glycosylated and contains immunoglobulin-like V-type domains critical for sialic acid binding, with multiple disulfide bonds stabilizing its structure.
• Keywords: 3D-structure, Alternative splicing, Cell adhesion, Cell membrane, Disulfide bond, Glycoprotein, Immunoglobulin domain, Isopeptide bond, Lectin, Membrane, Phosphoprotein, Proteomics identification, Reference proteome, Repeat, Signal, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is designed for flow cytometry applications; consider performing a titration to determine the optimal working concentration for your specific cell type.
• The immunogen covers the entire extracellular domain (aa 20-687), ensuring recognition of the native surface protein on live B-cells; validate in human B-lymphocyte cell lines or primary PBMCs.
• Use an appropriate isotype control (rabbit IgG) to assess nonspecific binding, and avoid using this antibody with patient samples undergoing anti-CD22 therapy if the therapeutic targets the same epitope region.
• No cross-reactivity data are available for species other than human; verify reactivity if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing bridge for IVD raw materials, providing validated antibody pairs, optimized monoclonal antibodies like this PE anti-human CD22 clone, and bulk ancillary reagents to streamline diagnostic development. This product supports B-cell immunophenotyping and lymphoma research applications, complementing our portfolio of rare-target raw materials. Our integrated technical and consulting services help accelerate your project from concept to clinic, ensuring reliable supply and performance.