PE Anti-Human CD171/L1CAM Monoclonal Antibody for Flow Cytometry - P32004

Core Product Specifications and Parameters

Biological Background: CD171/L1CAM Function and Localization

• L1CAM (neural cell adhesion molecule L1) is a transmembrane glycoprotein belonging to the immunoglobulin superfamily, characterized by multiple immunoglobulin-like domains and fibronectin type III repeats.
• It mediates both homophilic and heterophilic cell adhesion, playing a central role in the dynamic regulation of cell–cell interactions and transmembrane signaling at tyrosine kinase receptors.
• During brain development, L1CAM is critical for neuronal migration, axonal growth and fasciculation, and synaptogenesis, guiding the formation of functional neural circuits.
• In the mature brain, it contributes to synaptic plasticity and the maintenance of neuronal structure, influencing learning and memory.
• Subcellularly, L1CAM is localized to the cell membrane and is enriched in specialized regions such as growth cones, axons, and dendrites of neurons.
• Post-translational modifications, including phosphorylation, modulate L1CAM adhesive properties and signal transduction.
• Alternative splicing generates multiple L1CAM isoforms, potentially fine-tuning its functions in different tissues and developmental stages.
• Mutations in the L1CAM gene are associated with a spectrum of neurological disorders, including X-linked hereditary spastic paraplegia (SPG1), Hirschsprung disease, and X-linked intellectual disability (e.g., HSAS, MASA syndromes).

Experimental Guidance and Technical Tips

• This PE-conjugated antibody is intended for direct flow cytometric detection of CD171/L1CAM on human cells; validate staining conditions using appropriate positive and negative control cell lines.
• As a monoclonal antibody generated against a large recombinant extracellular domain (aa 20–1120), it likely recognizes an epitope within that region; consider epitope accessibility if fixation or permeabilization is required.
• For live-cell surface staining, maintain cells in cold buffer with azide to prevent antigen internalization and capping.
• Titrate the antibody to determine the optimal concentration for specific signal-to-noise ratio in your experimental system.
• Include matched PE-conjugated isotype controls at the same concentration to assess background staining.
• If combining with other fluorophores, verify spectral compatibility and compensation settings.
• Cross-reactivity has been validated only for human; for other species, confirm immunoreactivity independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials for neuroscience research and diagnostic development. In addition to the featured PE anti-L1CAM monoclonal antibody for flow cytometry, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing solutions to support your assay development from concept to clinic. Contact our team to discuss customized sourcing for your CD171/L1CAM detection needs.