PE Anti-Human CD15/SSEA-1 Monoclonal Antibody for Flow Cytometry - P22083

Core Product Specifications and Parameters

Biological Background: FUT4 (CD15/SSEA-1) Function and Localization

• FUT4, also known as fucosyltransferase IV (FucT-IV), is an alpha-(1,3)-fucosyltransferase that catalyzes the transfer of fucose to N-acetylglucosamine (GlcNAc) in type 2 lactosamine chains.
• Robustly fucosylates nonsialylated distal LacNAc units to form the Lewis X antigen (CD15), a key glycan determinant in development and immunity. Related references: PMID:1702034, PMID:1716630, PMID:29593094
• With lower efficiency, modifies sialylated LacNAc to generate sialyl Lewis X and 6-sulfo sialyl Lewis X determinants, which serve as selectin recognition epitopes. Related references: PMID:1716630, PMID:29593094
• Together with FUT7, contributes to selectin ligand biosynthesis and leukocyte trafficking.
• In certain cell types, may also fucosylate internal LacNAc units to create the VIM-2 antigen, another SELE epitope. Related references: PMID:11278338, PMID:1716630
• Does not generate Lewis X antigens.
• Localizes to the Golgi stack membrane.
• Expressed in bone marrow mesenchymal stem cells, cord blood promyelocytes, and peripheral blood myeloid and lymphoid cells.
• Keywords: Alternative initiation, Glycoprotein, Glycosyltransferase, Golgi apparatus, Inflammatory response, Membrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• This monoclonal antibody, generated using a synthetic peptide immunogen, is designed for flow cytometry (FC). Titrate the antibody to determine optimal working concentration for your specific cell type and experiment.
• Since CD15/SSEA-1 is a carbohydrate epitope synthesized by FUT4, ensure that your sample preparation preserves cell surface glycans. Consider using appropriate isotype controls and viability dyes.
• The PE conjugate is light-sensitive; handle and store protected from light. Avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and labs with validated antibodies, bulk reagents, and rare target supplies. This anti-CD15/SSEA-1 monoclonal antibody, targeting a key glycosylation antigen, exemplifies our commitment to providing quality tools for immunology and stem cell research. From monoclonal antibodies to technical consultation, CamelBio supports your workflow from concept to clinic.