PE Anti-CD94 Monoclonal Antibody for Flow Cytometry - O54707

Core Product Specifications and Parameters

Biological Background: CD94 Function and Localization

• CD94, encoded by the Klrd1 gene (also known as Killer cell lectin-like receptor subfamily D member 1), is a transmembrane protein that serves as a ligand-binding subunit in both inhibitory and activating immune receptor complexes on NK cells and subsets of T cells.
• In complex with KLRC1 (NKG2A), CD94 recognizes MHC-E loaded with self-peptides, transmitting inhibitory signals via ITIMs that recruit phosphatases to dampen activation signals, thus regulating NK cell cytotoxicity and preventing autoimmunity.
• In complex with KLRC2 (NKG2C), CD94 recognizes MHC-E loaded with MHC-G-derived peptides, transmitting activating signals via the adaptor DAP12, which plays a role in adaptive NK cell responses and maternal-fetal tolerance.
• CD94 functions as an immune checkpoint molecule; in tumor microenvironments with high MHC-E expression, the inhibitory KLRD1-KLRC1 complex can contribute to NK and T cell exhaustion, making it a target for cancer immunotherapy research.
• The protein localizes to the cell membrane and is characterized by a C-type lectin-like domain, disulfide bonds, glycosylation, and a transmembrane helix, features that are important for its structural integrity and ligand binding.
• CD94-mediated surveillance enables lymphocytes to monitor MHC class I expression, distinguishing healthy cells from those with downregulated MHC-I due to infection or transformation.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant fusion protein corresponding to the extracellular region (amino acids 59–179) of mouse CD94, suggesting recognition of the ligand-binding domain; ensure it is suitable for detecting cell surface CD94 in flow cytometry under non-permeabilizing conditions.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration, and include appropriate isotype controls and Fc receptor blocking to minimize non-specific binding to mouse immune cells.
• CD94 is expressed on NK cells and some T cell subsets; consider co-staining with markers such as NK1.1, CD3, or KLRG1 to resolve specific lymphocyte populations in mouse samples.
• Since CD94 can be co-expressed with inhibitory or activating partners, functional studies may benefit from combining this antibody with anti-NKG2A or anti-NKG2C to dissect immune checkpoint versus activation pathways.
• Fixation and permeabilization steps may alter epitope recognition; validate the antibody's performance under your specific experimental protocol, particularly if intracellular staining is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, offering reliable access to validated antibodies, recombinant proteins, and technical support from discovery to commercial scale. This PE-conjugated anti-CD94 monoclonal antibody supports immuno-oncology and NK cell biology research, addressing the growing need for immune checkpoint and adoptive cell therapy development. Whether you need bulk monoclonal antibodies, custom conjugation, or complementary assay reagents, CamelBio streamlines procurement to accelerate your diagnostic and therapeutic programs.