PDXK Rabbit mAb for WB, IF/ICC, ELISA - O00764

Core Product Specifications and Parameters

Biological Background: PDXK Function and Localization

• Pyridoxal kinase (PDXK) catalyzes the phosphorylation of dietary vitamin B6 vitamers—pyridoxal, pyridoxine, and pyridoxamine—to their active 5'-phosphate forms, including pyridoxal 5'-phosphate (PLP). PLP serves as an essential cofactor for over 140 enzymatic reactions. Related references: PMID:10987144, PMID:17766369, PMID:19351586
• The enzyme localizes predominantly to the cytoplasm and cytosol.
• PDXK is ubiquitously expressed, with notably high expression levels in the testis, including adult testis and spermatozoa. Related references: PMID:31187503, PMID:9099727
• PDXK is subject to post-translational regulation via acetylation and phosphorylation, and its activity depends on metal cofactors such as magnesium, manganese, zinc, cobalt, and sodium, consistent with its ATP-binding and kinase function.
• Mutations in PDXK are associated with Charcot-Marie-Tooth disease type 6C (HMSN6C), a peripheral neuropathy, highlighting its critical role in neuronal health.
• Alternative splicing gives rise to multiple transcript variants, and the protein is identified as a proteomics target in reference datasets.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 213–312 of human PDXK; this sequence may exhibit homology with mouse and rat orthologs, supporting the reported cross-reactivity. Researchers should verify recognition of the expected target in their model system.
• For Western blotting, PDXK has a predicted molecular weight of approximately 35 kDa, but multiple bands around 27–35 kDa may be observed due to isoform expression or post-translational modifications. Use appropriate loading controls and molecular weight markers.
• For immunofluorescence/immunocytochemistry, testis-derived cell lines or primary cells may serve as positive controls due to high PDXK expression. Optimize fixation and permeabilization conditions.
• In ELISA applications, consider using recombinant PDXK protein as a reference standard to establish sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical services, and consulting—from concept to clinic. This PDXK monoclonal antibody, along with validated antibody pairs, bulk ancillary reagents, and custom-target sourcing, supports your vitamin B6 metabolism and Charcot-Marie-Tooth disease research programs. Trust CamelBio as your single partner for scalable, quality-consistent IVD raw material supply.