PDK4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q16654

Core Product Specifications and Parameters

Biological Background: PDK4 Function and Localization

• PDK4, or [pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, is a mitochondrial kinase that phosphorylates and inactivates pyruvate dehydrogenase subunits PDHA1 and PDHA2, thereby regulating the tricarboxylic acid cycle and down-regulating aerobic respiration.
• By inhibiting pyruvate dehydrogenase, PDK4 shifts cellular metabolism from glucose utilization to fatty acid oxidation, playing a crucial role in metabolic homeostasis during prolonged fasting and starvation.
• PDK4 is involved in maintaining normal blood glucose levels, participating in the insulin signaling cascade, and preventing excessive ketone body accumulation under starved conditions.
• In the fed state, PDK4 mediates cellular responses to glucose levels and high-fat diet, balancing fatty acid oxidation and de novo fatty acid biosynthesis.
• Located in the mitochondrial matrix, PDK4 is ubiquitously expressed with highest levels in heart and skeletal muscle tissues.
• Additional roles for PDK4 include generation of reactive oxygen species, protection of detached epithelial cells against anoikis, and modulation of cell proliferation through its metabolic regulatory functions.

Experimental Guidance and Technical Tips

• The antibody was generated against a synthetic peptide from amino acids 312–411 of human PDK4. Users should verify immunoreactivity in their specific experimental systems, as this region may be conserved across species.
• For Western blotting, a band at approximately 46 kDa is expected. Consider using heart or skeletal muscle lysates as positive controls, given high PDK4 expression in these tissues.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval and antibody dilution will require empirical optimization. Tissues such as heart and skeletal muscle are recommended for initial testing.
• For ELISA applications, coating with recombinant PDK4 protein or the immunizing peptide may assist in confirming specificity. Cross-reactivity with other PDK isoforms should be assessed if relevant.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement platform for high-quality IVD raw materials. This anti-PDK4 antibody supports research into metabolic disorders, diabetes, and cancer metabolism, and is part of our extensive portfolio of polyclonal and monoclonal antibodies. Beyond antibodies, we provide validated antibody pairs, bulk ancillary reagents, and assistance with sourcing rare-target raw materials to accelerate your diagnostic development pipeline.