PDHA2 Rabbit Polyclonal Antibody (WB, IF/ICC, ELISA) - P29803

Core Product Specifications and Parameters

Biological Background: PDHA2 Function and Localization

• PDHA2 is the testis-specific alpha subunit of pyruvate dehydrogenase (PDHE1-A type II), essential for energy production in sperm. Related references: PMID:14638692
• It partners with PDHB to form the E1 heterotetramer, catalyzing TPP-dependent decarboxylation of pyruvate and reductive acetylation of lipoyl-E2. Related references: PMID:16436377
• The enzyme uses thiamine pyrophosphate (TPP) as a cofactor and requires magnesium ions for activity.
• Located in the mitochondrial matrix, consistent with its role in central carbon metabolism.
• Expression is restricted to postmeiotic spermatogenic cells in the testis, highlighting its specialized metabolic function in sperm.
• Post-translational modifications include phosphorylation, which regulates enzymatic activity.
• Keywords: Carbohydrate metabolism, Glucose metabolism, Mitochondrion, Oxidoreductase, Tricarboxylic acid cycle.

Experimental Guidance and Technical Tips

• The immunogen spans a large region of PDHA2 (aa 119–388), which may provide robust detection across isoforms.
• For WB, testis lysates or sperm extracts from human, mouse, or rat are appropriate positive controls.
• Consider optimizing antibody dilution for IF/ICC, as mitochondrial proteins may require permeabilization protocols.
• Validate cross-reactivity in species of interest, as sequence homology may influence binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with comprehensive IVD raw material access for mitochondrial metabolism and reproductive biology targets. We offer validated antibody pairs, optimized polyclonal antibodies like anti-PDHA2, and bulk ancillary reagents. Whether for routine assay development or rare target sourcing, CamelBio streamlines your procurement from concept to clinic.