PDE11A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9HCR9

Core Product Specifications and Parameters

Biological Background: PDE11A Function and Localization

• PDE11A (dual 3',5'-cyclic-AMP and -GMP phosphodiesterase 11A) is a dual-specificity enzyme that catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, regulating intracellular cyclic nucleotide concentrations and signal transduction.
  Related references: PMID:10725373, PMID:10906126, PMID:11050148

• Encoded by the PDE11A gene, alternative splicing generates multiple protein isoforms with distinct tissue expression patterns.

• The protein is localized predominantly in the cytoplasm/cytosol.

• Isoform 1 is detected in prostate, pituitary, heart, and liver, while isoform 4 is present in the adrenal cortex; isoform 2 may be expressed in the testis. PDE11A is not found in testis or penis at the protein level for isoform 1, explaining the weak inhibition by Tadalafil.

• Keywords associated with PDE11A include Allosteric enzyme, cAMP, cGMP, Cushing syndrome, Cytoplasm, Hydrolase, Metal-binding, Phosphoprotein, indicating its regulatory roles and disease relevance.

• The molecular weight of the canonical isoform is approximately 105 kDa (UniProt: 104,752 Da).

Experimental Guidance and Technical Tips

• The immunogen (aa 712–933 of human PDE11A) targets a region conserved in mouse and rat, supporting observed cross-reactivity with these species. Validate in your specific sample system.

• For Western blotting, expect a band near 105 kDa; consider using appropriate protein ladders and loading controls.

• In immunofluorescence/ICC, confirm subcellular localization in the cytoplasm of relevant cell types, according to published data.

• ELISA can be employed for quantitative detection; optimize coating and detection conditions experimentally.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-PDE11A antibody supports research into cAMP/cGMP signaling and related disorders, and we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development.