PDCD2L Rabbit pAb for WB, IHC-P, ELISA - Q9BRP1

Core Product Specifications and Parameters

Biological Background: PDCD2L Function and Localization

• PDCD2L, also known as Programmed cell death protein 2-like, functions as a uS5 assembly chaperone, likely facilitating the incorporation of ribosomal protein uS5 into the small ribosomal subunit during ribosome biogenesis.
• Its chaperone activity appears redundant with PDCD2, indicating overlapping roles in the ribosome assembly pathway.
• Localizes primarily to the nucleolus and nucleus, with additional presence in the cytosol, consistent with its involvement in ribosomal subunit assembly and transport.
• Exhibits higher expression in tissues such as lung, colon, mammary gland, cervix, stomach, and small intestine, suggesting tissue-specific regulation of ribosome biogenesis.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its chaperone activity or subcellular localization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human PDCD2L (1-358), raising a polyclonal antibody that recognizes multiple epitopes; consider this for consistent detection across applications.
• For Western blot, the predicted molecular weight is approximately 39 kDa; validate expression in your system using appropriate controls.
• In IHC-P, antigen retrieval conditions may require optimization; a standard citrate buffer (pH 6.0) protocol is a reasonable starting point.
• For ELISA, the antibody can be used for antigen capture or detection; pairing with a monoclonal antibody may enhance specificity.
• Lot-to-lot variability is inherent to polyclonal antibodies; if switching lots, re-optimize working concentrations in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, supplying high-quality antibodies like the anti-PDCD2L polyclonal antibody for ribosome biogenesis research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can source rare targets to streamline your diagnostic development from concept to clinic.