PDAP1 Rabbit pAb for WB, IF/ICC, ELISA - Q13442

Core Product Specifications and Parameters

Biological Background: PDAP1 Function and Localization

• PDAP1 (PDGF-associated protein 1), also known as PAP, PAP1, and HASPP28, is a 20630 Da heat- and acid-stable phosphoprotein encoded by the PDAP1 gene in humans.
• It functions as a modulator of PDGF signaling: it enhances PDGFA-stimulated cell growth in fibroblasts while inhibiting the mitogenic effect of PDGFB.
• PDAP1 is subject to multiple post-translational modifications, including acetylation, methylation, phosphorylation, and ubiquitination (isopeptide bond formation).
• The protein has been identified in proteome-wide identification studies, indicating its broad cellular expression.
• Its heat- and acid-stable properties suggest a role in stress responses or stable protein interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human PDAP1; consider verifying specificity for target detection in your sample system.
• Western blotting (WB): Expect a band around 21 kDa; optimization of blocking and washing conditions may improve signal-to-noise ratio.
• Immunofluorescence/ICC: Validate fixation and permeabilization protocols to ensure epitope accessibility.
• ELISA: Determine optimal coating antigen concentration and antibody dilution for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement solution for IVD raw materials, supplying validated antibody pairs, bulk ancillary reagents, and specialty targets such as anti-PDAP1 antibodies. For researchers focused on PDGF signaling and cell growth regulation studies, our one-stop service ensures reliable access to critical reagents, supporting your project from concept to clinic.