PCNP Rabbit pAb for WB, ELISA - Q8WW12

Core Product Specifications and Parameters

Biological Background: PCNP Function and Localization

• PCNP (PEST proteolytic signal-containing nuclear protein) is a nuclear protein predicted to participate in cell cycle progression.
• The protein contains a PEST sequence motif, typically associated with rapid intracellular degradation and regulatory protein turnover.
• Encoded by the PCNP gene, it belongs to the reference proteome set, highlighting its evolutionary conservation.
• Subcellular localization studies indicate predominant nuclear accumulation, consistent with its functional context.
• Post‑translational modifications include acetylation, phosphorylation, and ubiquitination, which may modulate its stability and activity.
• Alternative splicing generates multiple transcript variants, potentially diversifying functional isoforms.

Experimental Guidance and Technical Tips

• As a polyclonal antibody raised against full‑length human PCNP, it is likely to recognize multiple epitopes, which may enhance detection sensitivity in denaturing WB.
• For WB, consider a starting primary antibody dilution of 1:500–1:2000 and verify against the predicted 19 kDa band in the relevant sample system.
• In ELISA, the antibody can be used as either capture or detection reagent; perform checkerboard titrations to optimize pairing.
• Cross‑reactivity with mouse and rat enables translational studies, but always validate specificity in the target species due to potential sequence divergence.
• Because PCNP is a low‑abundance nuclear protein, enrich nuclear fractions or use extended exposure for clear signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers the PCNP Rabbit pAb as part of a comprehensive IVD raw‑material portfolio for cell cycle and oncology research. We provide validated antibody pairs, high‑purity monoclonal and polyclonal reagents, and ancillary bulk supplies to support your assay development. Partner with CamelBio for streamlined, end‑to‑end sourcing from concept to clinic.