PBXIP1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q96AQ6

Core Product Specifications and Parameters

Biological Background: PBXIP1 Function and Localization

• PBXIP1 (Pre-B-cell leukemia transcription factor-interacting protein 1), also known as hematopoietic PBX-interacting protein (HPIP), is a regulator of pre-B-cell leukemia transcription factors (PBXs).
• It inhibits the binding of the PBX1-HOX complex to DNA, thereby blocking the transcriptional activity of oncogenic E2A-PBX1 fusion proteins.
• PBXIP1 tethers estrogen receptor-alpha (ESR1) to microtubules, linking microtubule dynamics to estrogen receptor signaling.
• The protein is localized in both the cytoplasm and nucleus, and associates with the cytoskeleton and microtubule network.
• It is expressed in early hematopoietic precursors, indicating a role in blood cell development.
• Alternative splicing produces isoforms; the protein contains a coiled-coil domain and is regulated by phosphorylation.
• UniProt keywords: alternative splicing, coiled coil, cytoplasm, cytoskeleton, microtubule, nucleus, phosphoprotein, proteomics identification, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–190 of human PBXIP1, a region that may be conserved across splice variants; consider verifying isoform detection if needed.
• For Western blot (WB), a band near 81 kDa is anticipated; titration of antibody dilution may be required for optimal signal.
• In immunohistochemistry (IHC-P), test on relevant paraffin-embedded tissue sections (e.g., bone marrow or early hematopoietic tissues) with appropriate antigen retrieval and controls.
• For ELISA, pairing this antibody as capture or detection with matched antibodies may be optimized; include standardized positive and negative controls.
• Cross-reactivity with mouse and rat is reported; validate performance in the specific model system under study.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to high-quality IVD raw materials, including validated antibody pairs, bulk recombinant proteins, and ancillary reagents. For PBXIP1-related research in leukemia, hormone signaling, or developmental biology, we supply this polyclonal antibody alongside complementary products to accelerate your assay development. Our team supports customized sourcing for rare targets and scalable supply to meet your program needs.