PAX4 Rabbit Polyclonal Antibody for WB, ELISA - O43316

Core Product Specifications and Parameters

Biological Background: PAX4 Function and Localization

• PAX4 is a member of the paired box (PAX) family of transcription factors, characterized by the paired domain and homeobox DNA-binding motifs.
• It plays a central role in the differentiation and development of pancreatic beta cells, which produce insulin (By similarity).
• PAX4 mainly functions as a transcriptional repressor, binding to common elements in the glucagon, insulin, and somatostatin promoters to inhibit their expression (By similarity).
• It competes with PAX6 for the same promoter binding sites, thereby modulating endocrine cell fate (By similarity).
• PAX4 prevents differentiation of pancreatic alpha cells by repressing the expression of ARX, a key alpha cell transcription factor (By similarity).
• In the intestine, PAX4 drives enterochromaffin cell differentiation while repressing other enteroendocrine cell types through ARX repression (PubMed:37883554). Related references: PMID:37883554
• An alternative splicing isoform (Isoform 2) acts as a dominant negative, antagonizing PAX4 transcriptional activity.
• PAX4 localizes to the nucleus and associates with chromosomes, consistent with its role as a transcription factor. Mutations in PAX4 are associated with diabetes mellitus (MODY9).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 50-200 of human PAX4, encompassing part of the paired domain. This design may recognize both full-length and dominant-negative isoforms; verify isoform specificity in your system.
• For western blotting, expect a band around 38 kDa (predicted molecular weight). Optimize loading and transfer conditions for nuclear proteins.
• Consider using pancreatic beta cell lines (e.g., EndoC-βH1) or intestinal organoids as positive controls, given PAX4’s restricted expression.
• In ELISA applications, a recombinant PAX4 protein standard can assist in quantitation; ensure the assay buffer maintains nuclear protein solubility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between diagnostic developers and high-quality IVD raw materials. For researchers focused on pancreatic development and endocrine disorders, we offer not only this PAX4 antibody but also a full portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our sourcing expertise extends to rare targets, helping you streamline your assay development from concept to clinic.