PAX3 Rabbit Monoclonal Antibody for WB, ELISA - P23760

Core Product Specifications and Parameters

Biological Background: PAX3 Function and Localization

• PAX3 is a member of the paired box (PAX) family of transcription factors, characterized by a paired domain and a homeodomain that mediate sequence-specific DNA binding.
• It plays an essential role in neural crest development, governing the migration and differentiation of cells that form melanocytes, enteric ganglia, and craniofacial structures.
• PAX3 is critical for myogenesis, controlling early muscle lineage commitment and activating myogenic regulatory factors.
• Localized in the nucleus, PAX3 functions as a transcriptional activator; it cooperates with SOX10 to directly activate the MITF promoter, a master regulator of melanocyte development. Related references: PMID:21965087
• Mutations in the PAX3 gene cause Waardenburg syndrome types 1 and 3, characterized by sensorineural hearing loss and pigmentation abnormalities.
• PAX3 undergoes alternative splicing, producing multiple isoforms, and its activity can be modulated by phosphorylation; chromosomal rearrangements involving PAX3 are linked to alveolar rhabdomyosarcoma, where it acts as a proto-oncogene.
• Additional keywords from UniProt include: deafness, disease variant, chromosomal rearrangement, alternative splicing, phosphoprotein, myogenesis, neurogenesis, and developmental protein.

Experimental Guidance and Technical Tips

• For Western blot detection of the 53 kDa PAX3 protein, use nuclear-rich lysates or whole cell extracts from cells expressing PAX3 (e.g., melanoma cell lines). A band near the predicted molecular weight should be observed; validate with a known positive control.
• The immunogen maps to the C-terminal region (amino acids 350–450); therefore, the antibody may recognize multiple isoforms if the epitope is conserved. Researchers should confirm isoform specificity in their system.
• In ELISA applications, consider using recombinant PAX3 protein or the immunizing peptide as a positive coating antigen to establish sensitivity and specificity.
• Although cross-reactivity with mouse and rat PAX3 is indicated, it is advisable to compare the target sequences across species, particularly within the immunogen region, and verify detection in the relevant sample matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. Our expertise in molecular immunoreagents, such as this anti-PAX3 monoclonal antibody, supports applications in developmental biology and oncology biomarker development. Whether you require bulk quantities, custom formulations, or rare-target sourcing, CamelBio serves as a reliable partner to accelerate your assay development from concept to clinic.