Pan Phospho-Tyrosine Rabbit mAb - Pan p-Y

Core Product Specifications and Parameters

Experimental Guidance and Technical Tips

• Given the immunogen’s design—a random peptide library phosphorylated around tyrosine—this antibody likely exhibits broad reactivity toward tyrosine-phosphorylated proteins. Consider confirming specificity using phospho-specific controls, phosphatase-treated lysates, and competitive inhibition with free phosphotyrosine.
• For Western blotting, validate detection with lysates from cells treated with pervanadate or growth factors (e.g., EGF) to enrich tyrosine phosphorylation. Optimize blocking and antibody dilution to minimize non-specific binding.
• In immunoprecipitation applications, the antibody may co-precipitate multiple tyrosine-phosphorylated targets; use stringent wash conditions and verify identification via mass spectrometry if needed.
• For ELISA, include both phosphorylated and non-phosphorylated peptide controls to establish signal-to-background ratios. Titrate the antibody empirically under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with essential IVD raw materials, including extensively validated phospho-specific antibodies like this anti-pan-phosphotyrosine clone. We support tyrosine phosphorylation studies with reliable, batch-tested reagents—from antibody pairs to bulk ancillary supplies—streamlining your workflow from early-stage development to assay finalization. Whether optimizing a routine Western blot or building a high-throughput ELISA, CamelBio’s sourcing expertise ensures you receive consistent, quality-assured materials tailored to your project requirements.