Pan Cadherin Rabbit Monoclonal Antibody (mAb) for WB, IHC-P, ELISA - P12830 / P19022 / P22223 / P33151 / P55283 / P39038

Core Product Specifications and Parameters

Biological Background: Pan Cadherin Function and Localization

• Cadherins are calcium-dependent cell adhesion proteins that mediate homophilic interactions, contributing to cell sorting and epithelial integrity. Related references: PMID:11976333
• CDH1 interacts with AMOTL2 to anchor radial actin fibers at cell junctions, facilitating cellular response to contractile forces (By similarity).
• It participates in early desmosome assembly by recruiting DSG2 and DSP to desmosome plaques. Related references: PMID:29999492
• Acts as a potent invasion suppressor and serves as a ligand for integrin alpha-E/beta-7.
• The E-cadherin CTF2 fragment promotes non-amyloidogenic degradation of Abeta precursors.
• Serves as a receptor for Listeria monocytogenes internalin A, facilitating bacterial uptake.
• Subcellularly localizes to adherens junctions, desmosomes, cell membrane, endosomes, and the Golgi.
• Expressed in epithelial tissues including skin, esophageal epithelium, and liver, as well as in granuloma macrophages. Related references: PMID:27760340, PMID:22294297, PMID:24220297, PMID:3263290

Experimental Guidance and Technical Tips

• The immunogen design, derived from a conserved region of cadherin-2, supports broad recognition of multiple classical cadherins; confirm reactivity with your specific cadherin of interest.
• For western blotting, a predominant band around 140 kDa is expected, though slight variations among family members may occur; validate molecular weight markers accordingly.
• In immunohistochemistry (IHC-P), consider appropriate antigen retrieval and validate staining in known positive control tissues (e.g., epithelial tissues).
• ELISA performance may require optimization of coating conditions and blocking steps; cross-reactivity with other cadherins should be assessed if single-target detection is desired.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with one-stop access to premium IVD raw materials, technical services, and consulting—from concept to clinic. For pan cadherin and cell adhesion research, we can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even rare-target raw materials to streamline your assay development and scale-up needs.