Pan-Akt Rabbit mAb for Western Blot, IP, IF, IHC-P, ELISA - P31749 / P31751 / Q9Y243

Core Product Specifications and Parameters

Biological Background: Pan-Akt Function and Localization

• AKT1 is one of three closely related serine/threonine kinases (AKT1/PKBα, AKT2/PKBβ, AKT3/PKBγ) that share high structural similarity and overlapping substrate specificities, collectively regulating critical cellular processes.
  Related references: PMID:11882383 PMID:15526160 PMID:15861136
• Acting downstream of PI3K, AKT mediates the signaling of insulin and growth factors (IGF1, EGF, PDGF) to control metabolism, cell growth, proliferation, survival, and angiogenesis.
  Related references: PMID:12176338 PMID:12964941 PMID:11882383 PMID:15526160 PMID:15861136
• AKT promotes cell survival by directly phosphorylating and inactivating pro-apoptotic factors, including MAP3K5/ASK1 (Ser83) and BAD, and by activating transcription factors such as CREB1 and NF-κB.
  Related references: PMID:11154276 PMID:10926925 PMID:9829964
• Through phosphorylation of TSC2 and DEPDC5/CASTOR1, AKT stimulates mTORC1 activity, promoting protein synthesis and inhibiting autophagy.
  Related references: PMID:12150915 PMID:12172553 PMID:31548394 PMID:33594058
• AKT phosphorylates FOXO1, FOXO3, and FOXO4, triggering their binding to 14-3-3 proteins and cytoplasmic retention, thereby suppressing the transcription of pro-apoptotic genes.
  Related references: PMID:10358075
• The protein localizes to the cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, facilitating context-dependent signaling; it is ubiquitously expressed and frequently overactivated in cancers such as prostate and breast carcinoma.

Experimental Guidance and Technical Tips

• The expected molecular weight for Pan-Akt is approximately 56 kDa; a single band or multiple bands corresponding to different isoforms may be observed depending on the cell line and treatment conditions.
• For Western blotting (WB), consider starting with a 1:1000 dilution and optimizing based on the target abundance in your sample.
• For immunoprecipitation (IP), use 2-5 µg of antibody per 500 µg of total protein lysate; validate with appropriate positive and negative controls.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), optimize fixation and permeabilization, and test dilutions between 1:50 and 1:200.
• ELISA applications may require pairing with a suitable detection antibody; ensure the antibody recognizes the native protein under assay conditions.
• Cross-reactivity has been reported for human, mouse, and rat Akt; if using other species, verify sequence homology in the immunogen region (residues 1-123 of human Akt1).
• The antibody recognizes all three AKT isoforms; for isoform-specific studies, confirm specificity with genetic controls or specific inhibitors.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supplying high-quality IVD raw materials to diagnostic manufacturers and research institutions worldwide. This Pan-Akt Rabbit mAb exemplifies our commitment to providing well-characterized antibodies for critical targets in cancer, metabolic, and cell signaling research. Beyond individual antibodies, CamelBio offers a comprehensive catalog of bulk reagents, validated antibody pairs, and custom sourcing services to streamline your assay development from concept to clinic.