PAK1 Rabbit Polyclonal Antibody for WB and ELISA - Q13153

Core Product Specifications and Parameters

Biological Background: PAK1 Function and Localization

• PAK1 (p21-activated kinase 1), also known as alpha-PAK or p65-PAK, is a serine/threonine-protein kinase encoded by the PAK1 gene on chromosome 11. Alternative splicing produces multiple isoforms.
• PAK1 plays a central role in cytoskeleton dynamics, cell adhesion, migration, proliferation, apoptosis, and mitosis by integrating signals from integrins and receptor tyrosine kinases (PubMed:10551809, PubMed:11896197, PubMed:12876277). Related references: PMID:10551809, PMID:11896197, PMID:12876277
• Activated by GTPases CDC42 and RAC1, PAK1 links these Rho-family GTPases to the JNK MAP kinase pathway, and also phosphorylates MAP2K1 to trigger downstream MAPK cascades (PubMed:8805275, PubMed:9528787). Related references: PMID:8805275, PMID:9528787
• In the actin cytoskeleton, PAK1 reorganizes stress fibers and focal adhesion complexes, and phosphorylates myosin light chain (MYL9/MLC2) to regulate actomyosin contractility (PubMed:9032240, PubMed:9395435). Related references: PMID:9032240, PMID:9395435
• PAK1 protects against apoptosis by directly phosphorylating BAD, and also phosphorylates RAF1, promoting its mitochondrial translocation and BAD phosphorylation (PubMed:11733498). Related references: PMID:11733498
• Subcellularly, PAK1 localizes to the cytoplasm, focal adhesions, lamellipodia, ruffle membranes, invadopodia, nucleoplasm, and centrosome, reflecting its diverse roles.
• PAK1 is overexpressed in gastric cancer cells and tissues, and contributes to cancer cell migration and invasion via RUFY3-mediated pathways (PubMed:25766321). Related references: PMID:25766321
• Additional functions include regulation of insulin secretion, chromatin remodeling, GABA(A) receptor synaptic stability, and dendritic spine formation, highlighting its pleiotropic roles (PubMed:22669945, PubMed:23260667). Related references: PMID:22669945, PMID:23260667

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 130-250 of human PAK1, a region that may be conserved across species; cross-reactivity with rat is confirmed, but additional species should be validated individually.
• For western blotting (WB), a band around 61 kDa is expected; however, alternative splicing and post-translational modifications may yield multiple bands. Consider using whole-cell lysates from human or rat cell lines known to express PAK1, such as HeLa or A431.
• For ELISA applications, titrate the antibody against the recombinant immunogen or native protein in a suitable matrix; optimal working dilutions should be determined empirically.
• Due to PAK1's role in apoptosis and migration, monitor cell culture conditions (e.g., serum starvation, confluence) as they may influence expression levels.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw-material supplier, CamelBio provides diagnostic manufacturers and research labs with high-quality antibodies like this anti-PAK1 polyclonal antibody, essential for studying cancer cell signaling and cytoskeletal dynamics. We offer one-stop access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare or challenging targets. Leverage CamelBio's expertise to streamline your project from concept to clinic.